Simple item page
 

Calcium carbonate-enriched Cucurbita maxima increases the relative expression level of the PCSK9 gene in the liver of ovariectomized rats

cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-4731-6243
cris.virtual.author-orcid0000-0002-0744-9033
cris.virtual.author-orcid0000-0002-0937-8427
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcidd951fe7e-3886-48a5-8661-b05d0c844622
cris.virtualsource.author-orcid968d3cb8-3b2b-46fe-a17f-373169a0738c
cris.virtualsource.author-orcid31a85399-18e5-444d-b0c6-96b441dd2551
dc.abstract.enThis study aimed to determine the effect of pumpkin (Cucurbita maxima) enriched with calcium carbonate on the PCSK9 gene relative expression level in an animal model of postmenopausal osteoporosis. The study was conducted in forty female Wistar rats, which were divided into five groups: a group fed a standard diet (C) and four ovariectomized groups fed a standard diet (OVX_C): a group receiving a calcium-free diet (OVX_DEF), a group receiving a calcium-deficient diet with the addition of unenriched C.maxima (OVX_P), and the OVX_PCaC group that was fed a diet with the addition of C. maxima enriched with calcium carbonate. After 12 weeks of the dietary intervention, the rats were sacrificed, and their tissues and blood were collected. Serum levels of inflammatory markers, antioxidants, and lipids were measured using enzyme-linked immunosorbent assay method. The PCSK9 gene relative expression level was assessed using quantitative PCR. It was found that ovariectomy decreased PCSK9 gene expression. Enriched C. maxima, particularly with calcium carbonate, increased PCSK9 expression above control levels. LDL and HDL cholesterol concentrations were affected by ovariectomy but not by diet modifications. A significant increase in serum C-reactive protein was observed after consuming unenriched C. maxima, as opposed to C. maxima enriched with calcium. However, after consuming both types of C. maxima, the concentration of cyclooxygenase 2 and lipoxygenase 1 in the serum decreased. These findings indicate that calcium-enriched C. maxima modulates PCSK9 expression, inflammation, and lipid metabolism, offering potential benefits in managing estrogen deficiency-related metabolic disturbances.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Żywienia Człowieka i Dietetyki
dc.affiliation.instituteKatedra Technologii Gastronomicznej i Żywności Funkcjonalnej
dc.contributor.authorWawrzyniak, Natalia
dc.contributor.authorMikołajczyk-Stecyna, Joanna
dc.contributor.authorGramza-Michałowska, Anna
dc.contributor.authorSuliburska, Joanna
dc.date.accessioned2025-12-17T08:52:49Z
dc.date.available2025-12-17T08:52:49Z
dc.date.issued2026
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,4
dc.description.numberMarch 2026
dc.description.points40
dc.description.volume35
dc.identifier.doi10.1016/j.phanu.2025.100467
dc.identifier.issn2213-4344
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/6424
dc.languageen
dc.relation.ispartofPharmaNutrition
dc.relation.pagesart. 100467
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enenriched pumpkin
dc.subject.enCucurbita maxima
dc.subject.enPCSK9 expression
dc.subject.enestrogen deficiency
dc.subject.enlipid metabolism
dc.subject.eninflammation
dc.titleCalcium carbonate-enriched Cucurbita maxima increases the relative expression level of the PCSK9 gene in the liver of ovariectomized rats
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume35