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Insights into the expression of DNA (de)methylation genes responsive to nitric oxide signaling in potato resistance to late blight disease

cris.lastimport.scopus2025-10-23T06:59:59Z
cris.virtual.author-orcid0000-0002-1624-0514
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cris.virtual.author-orcid0000-0003-2281-223X
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cris.virtualsource.author-orcid08471968-0a68-4f5f-a975-03aa3a75c3ac
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dc.abstract.enOur previous study concerning the pathogen-induced biphasic pattern of nitric oxide (NO) burst revealed that the decline phase and a low level of NO, due to S-nitrosoglutathione reductase (GSNOR) activity, might be decisive in the upregulation of stress-sensitive genes via histone H3/H4 methylation in potato leaves inoculated with avr P. infestans. The present study refers to the NO-related impact on genes regulating DNA (de)methylation, being in dialog with histone methylation. The excessive amounts of NO after the pathogen or GSNO treatment forced the transient upregulation of histone SUVH4 methylation and DNA hypermethylation. Then the diminished NO bioavailability reduced the SUVH4-mediated suppressive H3K9me2 mark on the R3a gene promoter and enhanced its transcription. However, we found that the R3a gene is likely to be controlled by the RdDM methylation pathway. The data revealed the time-dependent downregulation of the DCL3, AGO4, and miR482e genes, exerting upregulation of the targeted R3a gene correlated with ROS1 overexpression. Based on these results, we postulate that the biphasic waves of NO burst in response to the pathogen appear crucial in establishing potato resistance to late blight through the RdDM pathway controlling R gene expression.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Bioinżynierii
dc.affiliation.instituteKatedra Fizjologii Roślin
dc.contributor.authorDrozda, Andżelika
dc.contributor.authorKurpisz, Barbara
dc.contributor.authorGuan, Yufeng
dc.contributor.authorArasimowicz-Jelonek, Magdalena
dc.contributor.authorPlich, Jarosław
dc.contributor.authorJagodzik, Przemysław
dc.contributor.authorKuźnicki, Daniel
dc.contributor.authorFloryszak-Wieczorek, Jolanta
dc.date.access2025-08-27
dc.date.accessioned2025-08-27T11:00:58Z
dc.date.available2025-08-27T11:00:58Z
dc.date.copyright2022-12-02
dc.date.issued2022
dc.description.abstract<jats:p>Our previous study concerning the pathogen-induced biphasic pattern of nitric oxide (NO) burst revealed that the decline phase and a low level of NO, due to S-nitrosoglutathione reductase (GSNOR) activity, might be decisive in the upregulation of stress-sensitive genes <jats:italic>via</jats:italic> histone H3/H4 methylation in potato leaves inoculated with avr <jats:italic>P</jats:italic>. <jats:italic>infestans</jats:italic>. The present study refers to the NO-related impact on genes regulating DNA (de)methylation, being in dialog with histone methylation. The excessive amounts of NO after the pathogen or GSNO treatment forced the transient upregulation of histone <jats:italic>SUVH4</jats:italic> methylation and DNA hypermethylation. Then the diminished NO bioavailability reduced the SUVH4-mediated suppressive H3K9me2 mark on the <jats:italic>R3a</jats:italic> gene promoter and enhanced its transcription. However, we found that the <jats:italic>R3a</jats:italic> gene is likely to be controlled by the RdDM methylation pathway. The data revealed the time-dependent downregulation of the <jats:italic>DCL3</jats:italic>, <jats:italic>AGO4</jats:italic>, and <jats:italic>miR482e</jats:italic> genes, exerting upregulation of the targeted <jats:italic>R3a</jats:italic> gene correlated with <jats:italic>ROS1</jats:italic> overexpression. Based on these results, we postulate that the biphasic waves of NO burst in response to the pathogen appear crucial in establishing potato resistance to late blight through the RdDM pathway controlling <jats:italic>R</jats:italic> gene expression.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_research
dc.description.financecost12000,00
dc.description.if5,6
dc.description.points100
dc.description.versionfinal_published
dc.description.volume13
dc.identifier.doi10.3389/fpls.2022.1033699
dc.identifier.issn1664-462X
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4437
dc.identifier.weblinkhttps://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.1033699/full
dc.languageen
dc.pbn.affiliationagriculture and horticulture
dc.relation.ispartofFrontiers in Plant Science
dc.relation.pagesart. 1033699
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.ennitric oxide
dc.subject.enDNA (de)methylation genes
dc.subject.enR3a
dc.subject.enRdDM pathway
dc.subject.enPhytophthora infestans
dc.titleInsights into the expression of DNA (de)methylation genes responsive to nitric oxide signaling in potato resistance to late blight disease
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume13
project.funder.name2017/25/B/NZ9/00905