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Adaptation of a simple method to determine the total terpenoid content in needles of coniferous trees

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cris.virtual.author-orcid0000-0001-5915-079X
cris.virtual.author-orcid0000-0001-5564-7360
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cris.virtualsource.author-orcid700a7b38-3e22-4226-9ee6-fdba15d8c519
cris.virtualsource.author-orcid62199eda-fe32-456c-9935-2e55f2101f3a
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enIn ecological research, quantitative methods are often used to measure the total content of metabolites groups (i.e., phenols, carbohydrates). Until recently, there has been no simple and effective method to determine the total terpenoid content with satisfactory repeatability and sensitivity. The procedure proposed by Ghorai et al. (2012) requires the use of fresh plant material. That may be problematic when experimental units are located far from a laboratory. Our goals were to optimize the procedure, and to find the threshold of misestimation using the procedure adjusted to work with dried material. Needles of Pinus sylvestris were used to test the effect of changes in drying, grinding, storage, and extraction on determined total terpenoid content. All applied changes in material storage and grinding decreased the quantity of the terpenoids in needles. Only air-dried and ball-milled material produced similar results to those obtained with fresh material – can be recommended if the fresh material unattainable. Air-dried material may be stored for up to three months, but it resulted in greater variation and then greater sample size is needed. Lower sample mass and solvent volume have no impact on accuracy. Shorter extraction time, oven-drying or microwaving leads to unreliability of measurements.
dc.affiliationWydział Leśny i Technologii Drewna
dc.affiliation.instituteKatedra Hodowli Lasu
dc.contributor.authorŁukowski, Adrian
dc.contributor.authorJagiełło, Radosław
dc.contributor.authorRobakowski, Piotr
dc.contributor.authorAdamczyk, Dawid
dc.contributor.authorKarolewski, Piotr
dc.date.access2026-03-16
dc.date.accessioned2026-03-24T12:02:42Z
dc.date.available2026-03-24T12:02:42Z
dc.date.copyright2021-10-13
dc.date.issued2022
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if5,2
dc.description.numberJanuary 2022
dc.description.points100
dc.description.versionfinal_published
dc.description.volume314
dc.identifier.doi10.1016/j.plantsci.2021.111090
dc.identifier.eissn1873-2259
dc.identifier.issn0168-9452
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/7917
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0168945221002867
dc.languageen
dc.relation.ispartofPlant Science
dc.relation.pagesart. 111090
dc.rightsCC-BY-NC-ND
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.enterpenoids
dc.subject.enprocedure modification
dc.subject.enoptimization
dc.subject.enPinus sylvestris
dc.subject.enspectrophotometric methods
dc.titleAdaptation of a simple method to determine the total terpenoid content in needles of coniferous trees
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume314