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Optimization of the flow cytometry method of detection, quantification and qualification of microorganisms in carrot juice

cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-9477-8563
cris.virtual.author-orcid0000-0002-2728-1303
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid73a2e591-ad92-47ae-b1e4-3eb06ca9d58f
cris.virtualsource.author-orcid5d972c6f-0c5b-4eec-9570-3e11020688af
dc.abstract.enFresh, unpasteurized carrot juice is a popular element of the everyday diet of many consumers, and as such the matter of the juice's microbial safety remains an important one. Imaging flow cytometry (FCM) allows a fast enumeration and determination of cells, as well as their further differentiation. However, carrot juice is a difficult food product to analyze with the use of FCM due to interference from autofluorescence and the presence of plant debris. In this research, we aimed to obtain an effective and repeatable protocol for the preparation of carrot juice samples for FCM analysis. Through experimental and software-based means we successfully determined a reliable protocol for the preparation of fresh, unpasteurized carrot juice, which consisted of a sequence of filtering, centrifugation, enzyme treatment, and finally the implementation of the Machine Learning protocol for the best result.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Technologii Żywności Pochodzenia Roślinnego
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.contributor.authorRatajczak, Katarzyna
dc.contributor.authorJuzwa, Wojciech
dc.contributor.authorPiotrowska-Cyplik, Agnieszka
dc.date.access2024-09-24
dc.date.accessioned2024-10-09T09:53:50Z
dc.date.available2024-10-09T09:53:50Z
dc.date.copyright2024-07-24
dc.date.issued2024
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0.00
dc.description.if8,5
dc.description.number1 December 2024
dc.description.points200
dc.description.reviewreview
dc.description.versionfinal_published
dc.description.volume460, Part 2
dc.identifier.doi10.1016/j.foodchem.2024.140606
dc.identifier.issn0308-8146
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/1799
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0308814624022568?via%3Dihub
dc.languageen
dc.relation.ispartofFood Chemistry
dc.relation.pagesart. 140606
dc.rightsCC-BY-NC
dc.sciencecloudsend
dc.subject.enFood safety
dc.subject.enFood microbiology
dc.subject.enFlow cytometry
dc.titleOptimization of the flow cytometry method of detection, quantification and qualification of microorganisms in carrot juice
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume460