MOTS-c modulates pancreatic islet function in rats and pigs in vitro

cris.lastimport.scopus2025-10-23T07:00:25Z
cris.virtual.author-orcid0000-0002-7182-6905
cris.virtual.author-orcid0000-0003-0715-0223
cris.virtual.author-orcid0000-0002-0081-7585
cris.virtual.author-orcid0000-0002-0412-5578
cris.virtual.author-orcid0000-0002-8798-991X
cris.virtual.author-orcid0000-0001-9792-1145
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cris.virtualsource.author-orcid325904ea-6775-4d8a-b846-0104d9ec20ca
cris.virtualsource.author-orcid0c22ceb5-e5c6-4f5d-a0fd-8ec91a16efa9
cris.virtualsource.author-orcidfa9a05b6-07fa-47e8-ae3d-8929da6881f3
cris.virtualsource.author-orcid1d7e7a9f-dfd0-4ed2-9512-a9aa9cf4ba27
cris.virtualsource.author-orcid7d619370-fc16-4ebc-8bfa-6738a36749ee
cris.virtualsource.author-orcid1815dbc7-82a3-4ce5-8dc9-bbe17826792b
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enMOTS-c is a promising regulator of metabolism and energy homeostasis. While its effects have been studied in cell lines, our team aimed to investigate its influence on more complex structures—specifically, isolated pancreatic islets. We used two animal models: the rat, which is commonly studied, and the pig, which shares greater physiological similarities with humans. This study assessed the expression and secretion of insulin and glucagon, the expression of their receptors, cell viability, and cell death following MOTS-c treatment of the islets. Additionally, we examined how MOTS-c secretion is affected by different incubation media, such as the presence of free fatty acids, pancreatic hormones, and different glucose concentrations. The results indicate that MOTS-c impacts pancreatic islet physiology by, for example, reducing insulin and glucagon secretion and enhancing cell viability. Notably, the effects differed between the two species, which may be attributed to anatomical differences in their pancreatic islets or structural variations in rat and pig MOTS-c. These facts may lead to the conclusion that if MOTS-c may be helpful in human medicine, the pig model should be considered another valuable choice.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.contributor.authorBień, Jakub
dc.contributor.authorPruszyńska-Oszmałek, Ewa
dc.contributor.authorKołodziejski, Paweł
dc.contributor.authorLeciejewska, Natalia
dc.contributor.authorSzczepankiewicz, Dawid
dc.contributor.authorGrzęda, Emilia
dc.contributor.authorSassek, Maciej
dc.date.access2025-07-02
dc.date.accessioned2025-07-02T12:10:05Z
dc.date.available2025-07-02T12:10:05Z
dc.date.copyright2025-06-06
dc.date.issued2025
dc.description.abstract<jats:title>Abstract</jats:title> <jats:p>MOTS-c is a promising regulator of metabolism and energy homeostasis. While its effects have been studied in cell lines, our team aimed to investigate its influence on more complex structures—specifically, isolated pancreatic islets. We used two animal models: the rat, which is commonly studied, and the pig, which shares greater physiological similarities with humans. This study assessed the expression and secretion of insulin and glucagon, the expression of their receptors, cell viability, and cell death following MOTS-c treatment of the islets. Additionally, we examined how MOTS-c secretion is affected by different incubation media, such as the presence of free fatty acids, pancreatic hormones, and different glucose concentrations. The results indicate that MOTS-c impacts pancreatic islet physiology by, for example, reducing insulin and glucagon secretion and enhancing cell viability. Notably, the effects differed between the two species, which may be attributed to anatomical differences in their pancreatic islets or structural variations in rat and pig MOTS-c. These facts may lead to the conclusion that if MOTS-c may be helpful in human medicine, the pig model should be considered another valuable choice.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,1
dc.description.number1
dc.description.points100
dc.description.versionfinal_published
dc.description.volume163
dc.identifier.doi10.1007/s00418-025-02391-4
dc.identifier.eissn1432-119X
dc.identifier.issn0948-6143
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/3807
dc.identifier.weblinkhttps://link.springer.com/article/10.1007/s00418-025-02391-4#Abs1
dc.languageen
dc.relation.ispartofHistochemistry and Cell Biology
dc.relation.pagesart. 64
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.enMOTS-c
dc.subject.enPancreas
dc.subject.enPancreatic islets
dc.titleMOTS-c modulates pancreatic islet function in rats and pigs in vitro
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume163