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Altered Transcript Levels of MMP13 and VIT Genes in the Muscle and Connective Tissue of Pigs with Umbilical Hernia

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cris.virtual.author-orcid0000-0003-2539-9508
cris.virtual.author-orcid0000-0002-1041-3576
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cris.virtualsource.author-orcidb1f9951f-b018-4795-8cff-c7188dd69448
dc.abstract.enUmbilical hernia (UH) and inguinal hernia (IH) are among the most common defects in pigs, affecting their welfare and resulting in economic losses. In this study, we aimed to verify the association of previously reported differences in transcript levels of the ACAN, COL6A5, MMP13, and VIT genes with the occurrence of UH and IH. We examined mRNA levels in muscle and connective tissue from 68 animals—34 affected by UH and 34 controls. In a second cohort, we examined inguinal channel samples from 46 pigs (in four groups). We determined DNA methylation levels in muscle tissue for the UH and control animals. The transcript level of MMP13 changed in the UH cases, being upregulated and downregulated in muscle and connective tissue, respectively, and the VIT gene also showed an increased muscular mRNA level. The transcript of the ACAN gene significantly decreased in old pigs with IH. We further observed an increased DNA methylation level for one CpG site within the MMP13 gene in UH individuals. We conclude that these alterations in gene mRNA levels in the UH animals depend on the tissue and can sometimes be a consequence of, not a cause of, the affected phenotype.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.contributor.authorWozniak, Jakub
dc.contributor.authorLoba, Weronika
dc.contributor.authorWysocka, Alicja
dc.contributor.authorDzimira, Stanislaw
dc.contributor.authorPrzadka, Przemyslaw
dc.contributor.authorŚwitoński, Marek
dc.contributor.authorNowacka-Woszuk, Joanna
dc.date.access2025-06-24
dc.date.accessioned2025-10-07T12:21:47Z
dc.date.available2025-10-07T12:21:47Z
dc.date.copyright2023-10-01
dc.date.issued2023
dc.description.abstract<jats:p>Umbilical hernia (UH) and inguinal hernia (IH) are among the most common defects in pigs, affecting their welfare and resulting in economic losses. In this study, we aimed to verify the association of previously reported differences in transcript levels of the ACAN, COL6A5, MMP13, and VIT genes with the occurrence of UH and IH. We examined mRNA levels in muscle and connective tissue from 68 animals—34 affected by UH and 34 controls. In a second cohort, we examined inguinal channel samples from 46 pigs (in four groups). We determined DNA methylation levels in muscle tissue for the UH and control animals. The transcript level of MMP13 changed in the UH cases, being upregulated and downregulated in muscle and connective tissue, respectively, and the VIT gene also showed an increased muscular mRNA level. The transcript of the ACAN gene significantly decreased in old pigs with IH. We further observed an increased DNA methylation level for one CpG site within the MMP13 gene in UH individuals. We conclude that these alterations in gene mRNA levels in the UH animals depend on the tissue and can sometimes be a consequence of, not a cause of, the affected phenotype.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,8
dc.description.number10
dc.description.points100
dc.description.versionfinal_published
dc.description.volume14
dc.identifier.doi10.3390/genes14101903
dc.identifier.issn2073-4425
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5257
dc.identifier.weblinkhttp://www.mdpi.com/2073-4425/14/10/1903
dc.languageen
dc.relation.ispartofGenes
dc.relation.pagesart. 1903
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enumbilical hernia
dc.subject.eninguinal hernia
dc.subject.enpig
dc.subject.enDNA methylation
dc.subject.entranscript level
dc.titleAltered Transcript Levels of MMP13 and VIT Genes in the Muscle and Connective Tissue of Pigs with Umbilical Hernia
dc.title.volumeThis article belongs to the Special Issue Trends and Prospects in Pig Genomics and Genetics
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue10
oaire.citation.volume14