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Enhancing genetic diversity in Coffea arabica L. through induced mutagenesis

cris.lastimport.scopus2025-10-23T06:58:51Z
cris.virtual.author-orcid0000-0002-0102-0084
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cris.virtualsource.author-orcid51a5a68b-106b-4e9d-bd9b-79d15d3ec0c1
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dc.abstract.enInduced mutagenesis is a powerful tool for enhancing genetic diversity and introducing novel agronomic traits, particularly in annual seed crops. However, its application in horticultural crops like Coffea arabica remains limited. Genetic improvement in C. arabica is hindered by its narrow genetic base, long reproductive cycle and the rapid decline of wild relatives. This study evaluated the effects of ethyl methanesulfonate (EMS) and gamma irradiation on seed germination, seedling growth, flowering and fertility in C. arabica. Seedling assays revealed that low EMS concentrations (0.2–2 %) stimulated germination, while higher concentrations (4–6 %) and increasing gamma radiation doses (20–100 Gy) progressively reduced germination rates. Long-term monitoring of mature M1 plants showed that gamma irradiation induced stable mutant phenotypes such as dwarfism, altered leaf morphology and chlorophyll defects that persisted from seedling to reproductive stage. In contrast, EMS-treated M1 plants appeared phenotypically similar to wild-type controls. Both mutagens influenced flowering and seed set. Gamma irradiation (50 Gy) induced early flowering and increased sterility, while EMS caused delayed flowering with reduced sterility. Notably, the altered leaf phenotypes and chlorophyll defects were also observed in the M2 progeny, providing initial evidence of stable transmission of induced mutant traits. These findings highlight the importance of applying a range of mutagen doses to mitigate mutagen-induced infertility. They also show, for the first time, that gamma- and EMS-induced genetic variation in C. arabica can be stably transmitted, offering a promising strategy to broaden its narrow genetic base.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Metod Matematycznych i Statystycznych
dc.contributor.authorNkurunziza, Radisras
dc.contributor.authorJankowicz-Cieslak, Joanna
dc.contributor.authorBocianowski, Jan
dc.contributor.authorBhatnagar-Mathur, Pooja
dc.contributor.authorWerbrouck, Stefaan P.O.
dc.contributor.authorIngelbrecht, Ivan L.W.
dc.date.access2025-07-11
dc.date.accessioned2025-07-23T11:15:15Z
dc.date.available2025-07-23T11:15:15Z
dc.date.copyright2025-07-03
dc.date.issued2025
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,5
dc.description.numberSeptember 2025
dc.description.points70
dc.description.versionfinal_published
dc.description.volume43
dc.identifier.doi10.1016/j.cpb.2025.100514
dc.identifier.issn2214-6628
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/3941
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S2214662825000829
dc.languageen
dc.relation.ispartofCurrent Plant Biology
dc.relation.pagesart. 100514
dc.rightsCC-BY-NC
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.encoffee
dc.subject.enmutation-assisted breeding
dc.subject.enmutant populations
dc.subject.engenetic variability
dc.subject.enfertility
dc.subject.enheritable mutations
dc.titleEnhancing genetic diversity in Coffea arabica L. through induced mutagenesis
dc.typeJournalArticle
dspace.entity.typePublication