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  4. DArTseq-Based, High-Throughput Identification of Novel Molecular Markers for the Detection of Blackleg (Leptosphaeria Spp.) Resistance in Rapeseed
 
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DArTseq-Based, High-Throughput Identification of Novel Molecular Markers for the Detection of Blackleg (Leptosphaeria Spp.) Resistance in Rapeseed

Type
Journal article
Language
English
Date issued
2024
Author
Starosta, Ewa
Jamruszka, Tomasz 
Szwarc, Justyna 
Bocianowski, Jan 
Jędryczka, Małgorzata
Grynia, Magdalena
Niemann, Janetta 
Faculty
Wydział Rolnictwa, Ogrodnictwa i Biotechnologii
Journal
International Journal of Molecular Sciences
ISSN
1422-0067
DOI
10.3390/ijms25158415
Web address
https://www.mdpi.com/1422-0067/25/15/8415
Volume
25
Number
15
Pages from-to
art. 8415
Abstract (EN)
Blackleg disease, caused by Leptosphaeria spp. fungi, is one of the most important diseases of Brassica napus, responsible for severe yield losses worldwide. Blackleg resistance is controlled by major R genes and minor quantitative trait loci (QTL). Due to the high adaptation ability of the pathogen, R-mediated resistance can be easily broken, while the resistance mediated via QTL is believed to be more durable. Thus, the identification of novel molecular markers linked to blackleg resistance for B. napus breeding programs is essential. In this study, 183 doubled haploid (DH) rapeseed lines were assessed in field conditions for resistance to Leptosphaeria spp. Subsequently, DArTseq-based Genome-Wide Association Study (GWAS) was performed to identify molecular markers linked to blackleg resistance. A total of 133,764 markers (96,121 SilicoDArT and 37,643 SNP) were obtained. Finally, nine SilicoDArT and six SNP molecular markers were associated with plant resistance to Leptosphaeria spp. at the highest significance level, p < 0.001. Importantly, eleven of these fifteen markers were found within ten genes located on chromosomes A06, A07, A08, C02, C03, C06 and C08. Given the immune-related functions of the orthologues of these genes in Arabidopsis thaliana, the identified markers hold great promise for application in rapeseed breeding programs.
Keywords (EN)
  • blackleg resistance

  • Leptosphaeria spp.

  • Leptosphaeria maculans

  • Leptosphaeria biglobosa

  • molecular markers

  • GWAS

  • SNP

  • DArTseq

  • next-generation sequencing

License
cc-bycc-by CC-BY - Attribution
Open access date
August 1, 2024
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