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DArTseq-Based, High-Throughput Identification of Novel Molecular Markers for the Detection of Blackleg (Leptosphaeria Spp.) Resistance in Rapeseed

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cris.virtual.author-orcid0000-0003-4445-5624
cris.virtual.author-orcid0000-0002-1018-1349
cris.virtual.author-orcid0000-0002-0102-0084
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cris.virtual.author-orcid0000-0002-8011-9487
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cris.virtualsource.author-orcid36fc7bfc-89c8-4648-af52-05dd31161d2f
cris.virtualsource.author-orcid296b0f8d-c4fe-4414-ac21-aad79119f2a0
cris.virtualsource.author-orcid51a5a68b-106b-4e9d-bd9b-79d15d3ec0c1
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cris.virtualsource.author-orcid20597688-8be2-4b58-9e15-29f5ff8c53aa
dc.abstract.enBlackleg disease, caused by Leptosphaeria spp. fungi, is one of the most important diseases of Brassica napus, responsible for severe yield losses worldwide. Blackleg resistance is controlled by major R genes and minor quantitative trait loci (QTL). Due to the high adaptation ability of the pathogen, R-mediated resistance can be easily broken, while the resistance mediated via QTL is believed to be more durable. Thus, the identification of novel molecular markers linked to blackleg resistance for B. napus breeding programs is essential. In this study, 183 doubled haploid (DH) rapeseed lines were assessed in field conditions for resistance to Leptosphaeria spp. Subsequently, DArTseq-based Genome-Wide Association Study (GWAS) was performed to identify molecular markers linked to blackleg resistance. A total of 133,764 markers (96,121 SilicoDArT and 37,643 SNP) were obtained. Finally, nine SilicoDArT and six SNP molecular markers were associated with plant resistance to Leptosphaeria spp. at the highest significance level, p < 0.001. Importantly, eleven of these fifteen markers were found within ten genes located on chromosomes A06, A07, A08, C02, C03, C06 and C08. Given the immune-related functions of the orthologues of these genes in Arabidopsis thaliana, the identified markers hold great promise for application in rapeseed breeding programs.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Genetyki i Hodowli Roślin
dc.affiliation.instituteKatedra Metod Matematycznych i Statystycznych
dc.contributor.authorStarosta, Ewa
dc.contributor.authorJamruszka, Tomasz
dc.contributor.authorSzwarc, Justyna
dc.contributor.authorBocianowski, Jan
dc.contributor.authorJędryczka, Małgorzata
dc.contributor.authorGrynia, Magdalena
dc.contributor.authorNiemann, Janetta
dc.date.access2024-08-22
dc.date.accessioned2024-08-22T10:51:29Z
dc.date.available2024-08-22T10:51:29Z
dc.date.copyright2024-08-01
dc.date.issued2024
dc.description.abstract<jats:p>Blackleg disease, caused by Leptosphaeria spp. fungi, is one of the most important diseases of Brassica napus, responsible for severe yield losses worldwide. Blackleg resistance is controlled by major R genes and minor quantitative trait loci (QTL). Due to the high adaptation ability of the pathogen, R-mediated resistance can be easily broken, while the resistance mediated via QTL is believed to be more durable. Thus, the identification of novel molecular markers linked to blackleg resistance for B. napus breeding programs is essential. In this study, 183 doubled haploid (DH) rapeseed lines were assessed in field conditions for resistance to Leptosphaeria spp. Subsequently, DArTseq-based Genome-Wide Association Study (GWAS) was performed to identify molecular markers linked to blackleg resistance. A total of 133,764 markers (96,121 SilicoDArT and 37,643 SNP) were obtained. Finally, nine SilicoDArT and six SNP molecular markers were associated with plant resistance to Leptosphaeria spp. at the highest significance level, p &lt; 0.001. Importantly, eleven of these fifteen markers were found within ten genes located on chromosomes A06, A07, A08, C02, C03, C06 and C08. Given the immune-related functions of the orthologues of these genes in Arabidopsis thaliana, the identified markers hold great promise for application in rapeseed breeding programs.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,9
dc.description.number15
dc.description.points140
dc.description.reviewreview
dc.description.versionfinal_published
dc.description.volume25
dc.identifier.doi10.3390/ijms25158415
dc.identifier.issn1422-0067
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/1677
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/25/15/8415
dc.languageen
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pagesart. 8415
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.enblackleg resistance
dc.subject.enLeptosphaeria spp.
dc.subject.enLeptosphaeria maculans
dc.subject.enLeptosphaeria biglobosa
dc.subject.enmolecular markers
dc.subject.enGWAS
dc.subject.enSNP
dc.subject.enDArTseq
dc.subject.ennext-generation sequencing
dc.titleDArTseq-Based, High-Throughput Identification of Novel Molecular Markers for the Detection of Blackleg (Leptosphaeria Spp.) Resistance in Rapeseed
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue15
oaire.citation.volume25
project.funder.nameb.d.