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Sodium Azide as a Chemical Mutagen in Wheat (Triticum aestivum L.): Patterns of the Genetic and Epigenetic Effects with iPBS and CRED-iPBS Techniques

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cris.virtual.author-orcid0000-0002-9670-3231
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cris.virtual.author-orcid0000-0002-1004-7254
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cris.virtual.author-orcid0000-0003-3721-6473
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dc.abstract.enWheat, which is scientifically known as Triticum aestivum L., is a very nutritious grain that serves as a key component of the human diet. The use of mutation breeding as a tool for crop improvement is a reasonably rapid procedure, and it generates a variety that may be used in selective breeding programs as well as functional gene investigations. The present experiment was used to evaluate the potential application of a conventional chemical mutagenesis technique via sodium azide (NaN3) for the germination and seedling growth stage in wheat. Experiments with NaN3 mutagenesis were conducted using four different treatment periods (0, 1, 2, and 3 h) and five different concentrations (0, 0.5, 1, 1.5, and 2 mM). The genomic instability and cytosine methylation of wheat using its seeds were investigated after they were treated. In order to evaluate the genomic instability and cytosine methylation in wheat that had been treated, interprimer binding site (iPBS) markers were used. The mutagenic effects of NaN3 treatments had considerable polymorphism on a variety of impacts on the cytosine methylation and genomic instability of wheat plants. The results of the experiment showed considerable changes in the iPBS profiles produced by the administration of the same treatments at different dosages and at different times. Coupled restriction enzyme digestion interprimer binding site (CRED-iPBS) assays identified changes in gDNA cytosine methylation. The highest polymorphism value was obtained during 1 h + 2 mM NaN3, while the lowest (20.7%) was obtained during 1 h + 1.5 mM NaN3. Results showed that treatments with NaN3 had an effect on the level of cytosine methylation and the stability of the genomic template in wheat plants in the germination stage. Additionally, an integrated method can be used to for mutation-assisted breeding using a molecular marker system in wheat followed by the selection of desired mutants
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliationWydział Inżynierii Środowiska i Inżynierii Mechanicznej
dc.affiliation.instituteKatedra Agronomii
dc.affiliation.instituteKatedra Gleboznawstwa i Mikrobiologii
dc.affiliation.instituteKatedra Inżynierii Biosystemów
dc.contributor.authorTürkoğlu, Aras
dc.contributor.authorHaliloğlu, Kamil
dc.contributor.authorTosun, Metin
dc.contributor.authorSzulc, Piotr
dc.contributor.authorDemirel, Fatih
dc.contributor.authorEren, Barış
dc.contributor.authorBujak, Henryk
dc.contributor.authorKaragöz, Halit
dc.contributor.authorSelwet, Marek
dc.contributor.authorÖzkan, Güller
dc.contributor.authorNiedbała, Gniewko
dc.date.access2025-05-27
dc.date.accessioned2025-08-29T06:44:45Z
dc.date.available2025-08-29T06:44:45Z
dc.date.copyright2023-06-14
dc.date.issued2023
dc.description.abstract<jats:p>Wheat, which is scientifically known as Triticum aestivum L., is a very nutritious grain that serves as a key component of the human diet. The use of mutation breeding as a tool for crop improvement is a reasonably rapid procedure, and it generates a variety that may be used in selective breeding programs as well as functional gene investigations. The present experiment was used to evaluate the potential application of a conventional chemical mutagenesis technique via sodium azide (NaN3) for the germination and seedling growth stage in wheat. Experiments with NaN3 mutagenesis were conducted using four different treatment periods (0, 1, 2, and 3 h) and five different concentrations (0, 0.5, 1, 1.5, and 2 mM). The genomic instability and cytosine methylation of wheat using its seeds were investigated after they were treated. In order to evaluate the genomic instability and cytosine methylation in wheat that had been treated, interprimer binding site (iPBS) markers were used. The mutagenic effects of NaN3 treatments had considerable polymorphism on a variety of impacts on the cytosine methylation and genomic instability of wheat plants. The results of the experiment showed considerable changes in the iPBS profiles produced by the administration of the same treatments at different dosages and at different times. Coupled restriction enzyme digestion interprimer binding site (CRED-iPBS) assays identified changes in gDNA cytosine methylation. The highest polymorphism value was obtained during 1 h + 2 mM NaN3, while the lowest (20.7%) was obtained during 1 h + 1.5 mM NaN3. Results showed that treatments with NaN3 had an effect on the level of cytosine methylation and the stability of the genomic template in wheat plants in the germination stage. Additionally, an integrated method can be used to for mutation-assisted breeding using a molecular marker system in wheat followed by the selection of desired mutants.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3,3
dc.description.number6
dc.description.points140
dc.description.versionfinal_published
dc.description.volume13
dc.identifier.doi10.3390/agriculture13061242
dc.identifier.issn2077-0472
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4496
dc.identifier.weblinkhttp://www.mdpi.com/2077-0472/13/6/1242
dc.languageen
dc.relation.ispartofAgriculture (Switzerland)
dc.relation.pagesart. 1242
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.engenomic template stability
dc.subject.enDNA methylation
dc.subject.enmutagen
dc.subject.eniPBS
dc.titleSodium Azide as a Chemical Mutagen in Wheat (Triticum aestivum L.): Patterns of the Genetic and Epigenetic Effects with iPBS and CRED-iPBS Techniques
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue6
oaire.citation.volume13