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Comparative evaluation of nine lateral flow assays for FIV antibody detection using an in-house ELISA as a reference method

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cris.virtual.author-orcid0000-0003-1630-2938
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cris.virtualsource.author-orcid527456dc-1715-40e8-bbcd-4761fce356aa
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dc.abstract.enFeline immunodeficiency virus (FIV) induces immunosuppression in affected cats, increasing susceptibility to chronic and secondary infections. Rapid and accurate detection of FIV-specific antibodies is essential for effective clinical management and epidemiological monitoring. This study conducted a comparative evaluation of nine commercially available lateral flow assays (LFAs) for detecting FIV antibodies in whole blood, serum, or plasma, using a newly developed in-house enzyme-linked immunosorbent assay (ELISA) as a reference method. All tested LFAs demonstrated 100% specificity. While Vet Expert new and VetFor achieved 100% across all metrics indicating the best performance, formal statistical comparison did not reveal significant differences between the evaluated kits. Overall, the results confirm that all tested LFAs offer comparable reliability. Importantly, our in-house ELISA exhibited 100% concordance for positive samples with the commercial ELISA treated as the reference standard, confirming its reliability as a comparator. These findings emphasize the importance of selecting high-performing diagnostic tools to ensure reliable FIV detection and effective disease control strategies.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Nauk Przedklinicznych i Chorób Zakaźnych
dc.contributor.authorLaska-Modzelewska, Alicja
dc.contributor.authorPawelczak, Paweł
dc.contributor.authorJank, Michał
dc.contributor.authorSkupińska, Mirosława
dc.contributor.authorBelter, Agnieszka
dc.date.access2026-02-20
dc.date.accessioned2026-02-20T10:11:45Z
dc.date.available2026-02-20T10:11:45Z
dc.date.copyright2026-01-23
dc.date.issued2026
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,0
dc.description.if2,6
dc.description.number1
dc.description.points140
dc.description.versionfinal_published
dc.description.volume22
dc.identifier.doi10.1186/s12917-026-05303-3
dc.identifier.issn1746-6148
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/7409
dc.identifier.weblinkhttps://link.springer.com/article/10.1186/s12917-026-05303-3#auth-Alicja-Laska_Modzelewska-Aff1
dc.languageen
dc.pbn.affiliationveterinary science
dc.relation.ispartofBMC Veterinary Research
dc.relation.pagesart. 110
dc.rightsCC-BY-NC-ND
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enFeline immunodeficiency virus
dc.subject.enRapid test
dc.subject.enLateral flow assay
dc.subject.enAntibodies
dc.subject.enAntigens
dc.subject.enEnzyme-linked immunosorbent assay
dc.titleComparative evaluation of nine lateral flow assays for FIV antibody detection using an in-house ELISA as a reference method
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume22