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Toxico-pathological effects of ochratoxin A and its diastereoisomer under in ovo conditions and in vitro evaluation of the toxicity of these toxins against the embryo Gallus gallus fibroblast cell line

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dc.abstract.enHerein, we conducted a comparative study on the embryotoxicity of ochratoxin A (OTA) and its diastereomer 2’R-ochratoxin A (2’R-OTA) under in ovo conditions, as well as assess the in vitro embryotoxicity of these substances together with ochratoxin B and α-ochratoxin, using chicken (Gallus gallus domesticus) embryo cell lines. In ovo tests involved egg incubation of 8 different groups (i.e., control “0”—no puncture or injection (standard incubation); “00”—punctured eggs without injection; “OTA 0.25,” “OTA 0.50,” “OTA 0.75,” “2’R-OTA 0.25,” “2’R-OTA 0.50,” “2’R-OTA 0.75”—eggs containing OTA or 2’R-OTA at 0.25, 0.50, and 0.75 µg/egg concentration, respectively). The results confirmed OTA's impact on early and late embryo mortality, where chick hatchability decreased with increasing toxin dosage. Both OTA and 2’R-OTA demonstrated embryotoxicity, however, in the case of the highest OTA diastereomer dose, nearly 11% higher chick hatchability was observed compared with the group that received OTA. 2’R-OTA dosage did not reduce parameters chick quality compared to chicks hatched from control group eggs. OTA concentrations were higher than 2’R-OTA detected in chicken organs such as liver and kidney, whereas 2’R-OTA concentrations were higher in blood serum and heart. The presented studies highlighted the differences in the ability to accumulate toxins in certain organs, which, to a certain extent, may affect the potential toxicity on individual organs. Additionally, during in vitro tests, when assessing the cytotoxic effects of OTA and its analogues toward the chicken embryonic cell line in an MTT assay, the cell metabolic activity was inhibited to a comparable extent at 27-times higher concentration of 2’R-OTA than OTA (0.24 µM). Also, comparably lower toxicity was attributed to the remaining OTA derivatives.
dc.affiliationWydział Leśny i Technologii Drewna
dc.affiliation.instituteKatedra Chemii
dc.contributor.authorBryła, Marcin
dc.contributor.authorDamaziak, Krzysztof
dc.contributor.authorTwarużek, Magdalena
dc.contributor.authorWaśkiewicz, Agnieszka
dc.contributor.authorStępień, Łukasz
dc.contributor.authorRoszko, Marek
dc.contributor.authorPierzgalski, Adam
dc.contributor.authorSoszczyńska, Ewelina
dc.contributor.authorŁukasiewicz-Mierzejewska, Monika
dc.contributor.authorChmiel, Marta
dc.contributor.authorWójcik, Wojciech
dc.date.access2024-09-12
dc.date.accessioned2024-10-23T07:50:29Z
dc.date.available2024-10-23T07:50:29Z
dc.date.copyright2022-12-08
dc.date.issued2023
dc.description.bibliographybibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3,8
dc.description.number2
dc.description.points140
dc.description.versionfinal_published
dc.description.volume102
dc.identifier.doi10.1016/j.psj.2022.102413
dc.identifier.issn0032-5791
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/1913
dc.languageen
dc.relation.ispartofPoultry Science
dc.relation.pagesart. 102413
dc.rightsCC-BY-NC-ND
dc.sciencecloudnosend
dc.subject.enochratoxin A
dc.subject.en2’R-ochratoxin A
dc.subject.enembryotoxicity
dc.subject.enGallus gallus
dc.titleToxico-pathological effects of ochratoxin A and its diastereoisomer under in ovo conditions and in vitro evaluation of the toxicity of these toxins against the embryo Gallus gallus fibroblast cell line
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue2
oaire.citation.volume102