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Response of Skin-Derived and Metastatic Human Malignant Melanoma Cell Lines to Thymoquinone and Thymoquinone-Loaded Liposomes

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dc.abstract.enThymoquinone has been proved to be effective against neoplasms, including skin cancer. Its high lipophilicity, however, may limit its potential use as a drug. Melanoma remains the deadliest of all skin cancers worldwide, due to its high heterogeneity, depending on the stage of the disease. Our goal was to compare the anti-cancer activity of free thymoquinone and thymoquinone-loaded liposomes on two melanoma cell lines that originated from different stages of this cancer: skin-derived A375 and metastatic WM9. We evaluated the proapoptotic effects of free thymoquinone by flow cytometry and Western blot, and its mitotoxicity by means of JC-1 assay. Additionally, we compared the cytotoxicity of free thymoquinone and thymoquinone in liposomes by WST-1 assay. Our results revealed a higher antiproliferative effect of TQ in WM9 cells, whereas its higher proapoptotic activity was observed in the A375 cell line. Moreover, the thymoquinone-loaded liposome was proved to exert stronger cytotoxic effect on both cell lines studied than free thymoquinone. Differences in the response of melanoma cells derived from different stages of the disease to thymoquinone, as well as their different responses to free and carrier-delivered thymoquinone, are essential for the development of new anti-melanoma therapies. However, further research is required to fully understand them.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Technologii Żywności Pochodzenia Roślinnego
dc.contributor.authorKłos, Patrycja
dc.contributor.authorPerużyńska, Magdalena
dc.contributor.authorBaśkiewicz-Hałasa, Magdalena
dc.contributor.authorSkupin-Mrugalska, Paulina
dc.contributor.authorMajcher, Małgorzata Anna
dc.contributor.authorSawczuk, Magdalena
dc.contributor.authorSzostak, Bartosz
dc.contributor.authorDroździk, Marek
dc.contributor.authorMachaliński, Bogusław
dc.contributor.authorChlubek, Dariusz
dc.date.access2026-03-25
dc.date.accessioned2026-03-25T11:56:09Z
dc.date.available2026-03-25T11:56:09Z
dc.date.copyright2022-10-27
dc.date.issued2022
dc.description.abstract<jats:p>Thymoquinone has been proved to be effective against neoplasms, including skin cancer. Its high lipophilicity, however, may limit its potential use as a drug. Melanoma remains the deadliest of all skin cancers worldwide, due to its high heterogeneity, depending on the stage of the disease. Our goal was to compare the anti-cancer activity of free thymoquinone and thymoquinone-loaded liposomes on two melanoma cell lines that originated from different stages of this cancer: skin-derived A375 and metastatic WM9. We evaluated the proapoptotic effects of free thymoquinone by flow cytometry and Western blot, and its mitotoxicity by means of JC-1 assay. Additionally, we compared the cytotoxicity of free thymoquinone and thymoquinone in liposomes by WST-1 assay. Our results revealed a higher antiproliferative effect of TQ in WM9 cells, whereas its higher proapoptotic activity was observed in the A375 cell line. Moreover, the thymoquinone-loaded liposome was proved to exert stronger cytotoxic effect on both cell lines studied than free thymoquinone. Differences in the response of melanoma cells derived from different stages of the disease to thymoquinone, as well as their different responses to free and carrier-delivered thymoquinone, are essential for the development of new anti-melanoma therapies. However, further research is required to fully understand them.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if5,4
dc.description.number11
dc.description.points100
dc.description.versionfinal_published
dc.description.volume14
dc.identifier.doi10.3390/pharmaceutics14112309
dc.identifier.issn1999-4923
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/7933
dc.identifier.weblinkhttps://www.mdpi.com/1999-4923/14/11/2309
dc.languageen
dc.relation.ispartofPharmaceutics
dc.relation.pagesart. 2309
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enthymoquinone
dc.subject.enmelanoma
dc.subject.enmetastasis
dc.subject.enliposomes
dc.subject.enapoptosis
dc.subject.encytotoxicity
dc.subject.enmitotoxicity
dc.titleResponse of Skin-Derived and Metastatic Human Malignant Melanoma Cell Lines to Thymoquinone and Thymoquinone-Loaded Liposomes
dc.title.volumeSpecial Issue Novel Strategies to Improve the Therapeutic Effects of Natural Anticancer Compounds
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue11
oaire.citation.volume14