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Biotechnological stimulation of phenolics in Lychnis flos-cuculi shoot cultures

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dc.abstract.enRagged Robin (Lychnis flos-cuculi L.) is a plant with notable medicinal potential, attributed to its wide spectrum of biological and pharmacological properties. Biotechnological methods were used for L. flos-cuculi to sustainably enhance the production of biomass and valuable secondary metabolites from a species with declining natural populations and promising pharmacological potential. The present study investigated the impact of biotechnological strategies-specifically, elicitation using Ganoderma lucidum (Reishi) extract and precursor feeding with L-phenylalanine (L-Phe)-on biomass growth and the accumulation of phenolic compounds in shoot cultures. These treatments were applied to clonally propagated, agitated shoot cultures. Chromatographic analyses confirmed the presence of species secondary metabolites and demonstrated that both elicitation and precursor feeding contributed to increased levels of key phenolic acids and flavonoids. Fungal extract elicitation and L-Phe supplementation enhanced the biosynthesis of several target compounds, with the effectiveness influenced by the duration of treatment and the concentration applied. Among the bioactive molecules stimulated were syringic acid, ferulic acid, caftaric acid, vitexin, and apigenin. Notably, precursor feeding with L-Phe promoted the accumulation of phenolic acids in a concentration-dependent manner and positively influenced flavonoid content, particularly vitexin and rutoside. These results support the application of elicitors and metabolic precursors as effective tools in plant biotechnology for optimizing the production of valuable secondary metabolites in L. flos-cuculi shoot cultures.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Warzywnictwa
dc.contributor.authorKikowska, Małgorzata
dc.contributor.authorHermosaningtyas, Anastasia
dc.contributor.authorGornowicz-Porowska, Justyna
dc.contributor.authorBudzianowska, Anna
dc.contributor.authorSiwulski, Marek
dc.contributor.authorBlicharska, Eliza
dc.contributor.authorThiem, Barbara
dc.contributor.authorUrbańska, Maria
dc.date.access2025-06-18
dc.date.accessioned2025-07-08T09:37:57Z
dc.date.available2025-07-08T09:37:57Z
dc.date.copyright2025-06-16
dc.date.issued2025
dc.description.abstract<jats:title>Abstract</jats:title> <jats:p>Ragged Robin (<jats:italic>Lychnis flos-cuculi</jats:italic> L.) is a plant with notable medicinal potential, attributed to its wide spectrum of biological and pharmacological properties. Biotechnological methods were used for <jats:italic>L. flos-cuculi</jats:italic> to sustainably enhance the production of biomass and valuable secondary metabolites from a species with declining natural populations and promising pharmacological potential. The present study investigated the impact of biotechnological strategies—specifically, elicitation using <jats:italic>Ganoderma lucidum</jats:italic> (Reishi) extract and precursor feeding with L-phenylalanine (L-Phe)—on biomass growth and the accumulation of phenolic compounds in shoot cultures. These treatments were applied to clonally propagated, agitated shoot cultures. Chromatographic analyses confirmed the presence of species secondary metabolites and demonstrated that both elicitation and precursor feeding contributed to increased levels of key phenolic acids and flavonoids. Fungal extract elicitation and L-Phe supplementation enhanced the biosynthesis of several target compounds, with the effectiveness influenced by the duration of treatment and the concentration applied. Among the bioactive molecules stimulated were syringic acid, ferulic acid, caftaric acid, vitexin, and apigenin. Notably, precursor feeding with L-Phe promoted the accumulation of phenolic acids in a concentration-dependent manner and positively influenced flavonoid content, particularly vitexin and rutoside. These results support the application of elicitors and metabolic precursors as effective tools in plant biotechnology for optimizing the production of valuable secondary metabolites in <jats:italic>L. flos-cuculi</jats:italic> shoot cultures.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,4
dc.description.number1
dc.description.points100
dc.description.versionfinal_published
dc.description.volume162
dc.identifier.doi10.1007/s11240-025-03117-z
dc.identifier.eissn1573-5044
dc.identifier.issn0167-6857
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/3821
dc.identifier.weblinkhttps://link.springer.com/article/10.1007/s11240-025-03117-z
dc.languageen
dc.pbn.affiliationagriculture and horticulture
dc.relation.ispartofPlant Cell, Tissue and Organ Culture
dc.relation.pagesart. 2
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOTHER
dc.subject.enragged robin
dc.subject.enagitated shoot culture
dc.subject.enelicitation with mycelium extract
dc.subject.enprecursor feeding
dc.subject.ensecondary metabolites
dc.subject.enphenolic compounds
dc.titleBiotechnological stimulation of phenolics in Lychnis flos-cuculi shoot cultures
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume162