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An assessment of the biotransformation and bioavailability of zearalenone and its modified forms using the Caco-2 cell line

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cris.virtual.author-orcid0000-0003-4113-1595
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dc.abstract.enZearalenone (ZEN) is a common Fusarium mycotoxin that is found in cereal products and has xenoestrogenic properties. ZEN's toxicity and interaction with the environment in vivo have been widely studied, but fewer data are available on its metabolites. This study aimed to evaluate the intestinal permeability of ZEN, its hydrogenated metabolites, and its glucosidic conjugates and qualitatively assess the metabolism of these compounds in the epithelium. A monolayer of Caco-2 cells was used as the bioavailability prediction model. The MTT cytotoxicity assay (metabolic activity assessment) was used to determine the concentrations of the test compound that do not reduce cell viability. Permeability coefficients were determined based on the compounds' concentrations in the acceptor compartment. Cell lysates were analyzed to obtain a qualitative assessment of the potential of ZEN metabolisation in epithelial cells. Quantitative and qualitative analyses were performed using LC-HRMS. The results indicate the efficient and variable intestinal permeability of ZEN and its hydrogenated metabolites. A lower permeability of the glucoside metabolites compared with the native forms was observed. Glucosidic conjugates were characterised by higher efflux ratios, suggesting more efficient intestinal reflux. Concerning the metabolism occurring in the intestinal epithelium model, the hydrolysis of ZEN derivatives and the release of native toxins, the bilateral conversion between ZEN and α-zearalenol (α-ZOL)/β-zearalenol (β-ZOL), and the presence of glucuronide metabolites were observed. Sulfated metabolites of α-ZOL and β-ZOL were also detected.
dc.affiliationWydział Leśny i Technologii Drewna
dc.affiliation.instituteKatedra Chemii
dc.contributor.authorPierzgalski, Adam
dc.contributor.authorPopowski, Dominik
dc.contributor.authorBryła, Marcin
dc.contributor.authorRoszko, Marek
dc.contributor.authorWaśkiewicz, Agnieszka
dc.date.access2025-11-18
dc.date.accessioned2025-11-18T10:20:11Z
dc.date.available2025-11-18T10:20:11Z
dc.date.copyright2025-08-05
dc.date.issued2025
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,4
dc.description.numberNovember 2025
dc.description.points100
dc.description.versionfinal_published
dc.description.volume266
dc.identifier.doi10.1016/j.toxicon.2025.108517
dc.identifier.eissn1879-3150
dc.identifier.issn0041-0101
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5951
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0041010125002922
dc.languageen
dc.relation.ispartofToxicon
dc.relation.pagesart. 108517
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.enzearalenone metabolites
dc.subject.enα-zearalenol
dc.subject.enβ-zearalenol
dc.subject.enintestinal permeability
dc.subject.enmycotoxins
dc.subject.enin vitro metabolism
dc.titleAn assessment of the biotransformation and bioavailability of zearalenone and its modified forms using the Caco-2 cell line
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume266