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Evaluation of the utility of testicular-only processing fluid for porcine reproductive and respiratory syndrome virus diagnostics and the effect of sample pooling on the test results

cris.lastimport.scopus2025-10-23T06:58:16Z
cris.virtual.author-orcid0000-0002-4509-8852
cris.virtual.author-orcid0000-0003-0270-2914
cris.virtual.author-orcid0000-0003-2220-2730
cris.virtualsource.author-orcid702c3b7e-b378-411f-bc9b-510fe73f43da
cris.virtualsource.author-orcidabb21f0b-d43c-470d-99e6-7b59a4439244
cris.virtualsource.author-orcid71dccebf-e765-40b9-87bb-e98ab3b7299c
dc.abstract.enIntroduction The testicular-only processing fluid (TOPF) obtained from piglet testicles after castration could be an alternative sample for porcine reproductive and respiratory syndrome (PRRS) laboratory diagnosis. If this matrix were proved useful, testing it would spare piglets the stress of blood drawing and eliminate some labour required to take blood samples. The aim of the study was to evaluate the utility of TOPF for this diagnostic purpose. Material and Methods Serum-and-TOPF pairs from male piglets and sera from female piglets were tested using commercial ELISA and real-time RT-PCR kits. For the pooling simulation, 10 μL aliquots of TOPF separated into low-, moderately and highly positive were mixed with appropriate volumes of negative TOPF samples. This simulated pools of 5, 10, 20, 40 and 80 samples containing 1 positive for serological analyses and pools of 10, 20, 40, 80, 160 and 320 samples containing 1 positive in molecular analyses. Results The percentages of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies were statistically significantly different (P-value < 0.05) between boar sera (69.55%) and TOPF (54.49%), as well as between gilt sera (74.52%) and TOPF. However, after adjusting the cut-off value, no significant differences were noted. The RNA of PRRSV was detected in 21.26% of male sera, 15.23% of TOPFs and 17.00% of female sera. Pooled sample testing revealed discrepancies in positive results associated with the pool size and original sample positivity strength. Conclusion TOPF samples can be a valuable matrix for laboratory PRRS diagnosis in piglets. However, it is important to be aware of the potential for false-negative results.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Nauk Przedklinicznych i Chorób Zakaźnych
dc.contributor.authorTurlewicz-Podbielska, Hanna
dc.contributor.authorDors, Arkadiusz
dc.contributor.authorPomorska-Mól, Małgorzata
dc.date.access2025-04-25
dc.date.accessioned2025-04-25T12:00:09Z
dc.date.available2025-04-25T12:00:09Z
dc.date.copyright2025-03-01
dc.date.issued2025
dc.description.abstract<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Introduction</jats:title> <jats:p>The testicular-only processing fluid (TOPF) obtained from piglet testicles after castration could be an alternative sample for porcine reproductive and respiratory syndrome (PRRS) laboratory diagnosis. If this matrix were proved useful, testing it would spare piglets the stress of blood drawing and eliminate some labour required to take blood samples. The aim of the study was to evaluate the utility of TOPF for this diagnostic purpose.</jats:p> </jats:sec> <jats:sec> <jats:title>Material and Methods</jats:title> <jats:p>Serum-and-TOPF pairs from male piglets and sera from female piglets were tested using commercial ELISA and real-time RT-PCR kits. For the pooling simulation, 10 μL aliquots of TOPF separated into low-, moderately and highly positive were mixed with appropriate volumes of negative TOPF samples. This simulated pools of 5, 10, 20, 40 and 80 samples containing 1 positive for serological analyses and pools of 10, 20, 40, 80, 160 and 320 samples containing 1 positive in molecular analyses.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The percentages of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies were statistically significantly different (P-value &lt; 0.05) between boar sera (69.55%) and TOPF (54.49%), as well as between gilt sera (74.52%) and TOPF. However, after adjusting the cut-off value, no significant differences were noted. The RNA of PRRSV was detected in 21.26% of male sera, 15.23% of TOPFs and 17.00% of female sera. Pooled sample testing revealed discrepancies in positive results associated with the pool size and original sample positivity strength.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>TOPF samples can be a valuable matrix for laboratory PRRS diagnosis in piglets. However, it is important to be aware of the potential for false-negative results.</jats:p> </jats:sec>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_research
dc.description.financecost3000,00
dc.description.if1,3
dc.description.number1
dc.description.points140
dc.description.versionfinal_published
dc.description.volume69
dc.identifier.doi10.2478/jvetres-2025-0011
dc.identifier.eissn2450-8608
dc.identifier.issn2450-7393
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2719
dc.identifier.weblinkhttps://sciendo.com/pl/article/10.2478/jvetres-2025-0011
dc.languageen
dc.pbn.affiliationveterinary science
dc.relation.ispartofJournal of Veterinary Research (Poland)
dc.relation.pages7-16
dc.relation.projectprojekt UMO-2020/37/N/NZ7/00084
dc.rightsCC-BY-NC-ND
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.endiagnostic matrices
dc.subject.enporcine reproductive and respiratory syndrome
dc.subject.enprocessing fluid
dc.subject.entesticular-only processing fluid
dc.subject.enpigs
dc.titleEvaluation of the utility of testicular-only processing fluid for porcine reproductive and respiratory syndrome virus diagnostics and the effect of sample pooling on the test results
dc.typeJournalArticle
dspace.entity.typePublication
project.funder.nameprojekt PREIDUB