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Molecular background of HAC1-driven improvement in the secretion of recombinant protein in Yarrowia lipolytica based on comparative transcriptomics

cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0001-8372-8459
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid6f5a4155-2edb-48cf-b362-f3180e151169
dc.abstract.enWhile the unfolded protein response (UPR) and its major regulator – transcription factor Hac1 are well-conserved across Eukarya, species-specific variations are repeatedly reported. Here we investigated molecular mechanisms by which co-over-expression of HAC1 improves secretion of a recombinant protein (r-Prot) in Yarrowia lipolytica, using comparative transcriptomics. Co-over-expression of HAC1 caused an >2-fold increase in secreted r-Prot, but its intracellular levels were decreased. The unconventional splicing rate of the HAC1 mRNA was counted through transcript sequencing. Multiple biological processes were affected in the HAC1-and-r-Prot co-over-expressing strain, including ribosome biogenesis, nuclear and mitochondrial events, cell cycle arrest, attenuation of gene expression by RNA polymerase III and II, as well as modulation of proteolysis and RNA metabolism; but whether the HAC1 co-over-expression/induction was the actual causative agent for these changes, was not always clear. We settled that the expression of the “conventional” HAC1 targets (KAR2 and PDI1) is not affected by its over-expression.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.contributor.authorKorpys-Woźniak, Paulina
dc.contributor.authorCelińska, Ewelina
dc.date.access2025-06-06
dc.date.accessioned2025-09-04T09:49:57Z
dc.date.available2025-09-04T09:49:57Z
dc.date.copyright2023-05-08
dc.date.issued2023
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.numberJune 2023
dc.description.points100
dc.description.versionfinal_published
dc.description.volume38
dc.identifier.doi10.1016/j.btre.2023.e00801
dc.identifier.issn2215-017X
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4626
dc.identifier.weblinkhttp://www.sciencedirect.com/science/article/pii/S2215017X23000218
dc.languageen
dc.relation.ispartofBiotechnology Reports
dc.relation.pagese00801
dc.rightsCC-BY-NC-ND
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.ensecretion
dc.subject.enheterologous protein
dc.subject.enyeast
dc.subject.enendoplasmic reticulum (ER) stress
dc.subject.enrecombinant protein production
dc.titleMolecular background of HAC1-driven improvement in the secretion of recombinant protein in Yarrowia lipolytica based on comparative transcriptomics
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume38