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Pulsed Electric Field (PEF) Treatment Results in Growth Promotion, Main Flavonoids Extraction, and Phytochemical Profile Modulation of Scutellaria baicalensis Georgi Roots

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dc.abstract.enThis study aims to explore the effect of pulsed electric field (PEF) treatment as a method very likely to result in reversible electroporation of Scutellaria baicalensis Georgi underground organs, resulting in increased mass transfer and secondary metabolites leakage. PEF treatment with previously established empirically tailored parameters [E = 0.3 kV/cm (U = 3 kV, d = 10 cm), t = 50 µs, N = 33 f = 1 Hz] was applied 1–3 times to S. baicalensis roots submerged in four different Natural Deep Eutectic Solvents (NADES) media (1—choline chloride/xylose (1:2) + 30% water, 2—choline chloride/glucose (1:2) + 30% water, 3—choline chloride/ethylene glycol (1:2), and 4—tap water (EC = 0.7 mS/cm). Confocal microscopy was utilized to visualize the impact of PEF treatment on the root cells in situ. As a result of plant cell membrane permeabilization, an extract containing major active metabolites was successfully acquired in most media, achieving the best results using medium 1 and repeating the PEF treatment twice (baicalein <LOQ, baicalin 12.85 µg/mL, wogonin 2.15 µg/mL, and wogonoside 3.01 µg/mL). Wogonin concentration in NADES media was on par with the control (plants harvested on the day of the experiment, ultrasound-mediated methanolic extraction, Cwogonin = 2.15 µg/mL). After successful extraction, PEF treatment allowed the plants to continue growing, with the lowest survival rate across treated groups being 60%. Additionally, an enhancement in plant growth parameters (length and fresh mass of the roots) and significant changes in the S. baicalensis root phytochemical profile were also observed.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.affiliation.instituteKatedra Żywienia Zwierząt
dc.affiliation.instituteKatedra Hodowli Zwierząt i Oceny Surowców
dc.contributor.authorGrzelka, Kajetan
dc.contributor.authorMatkowski, Adam
dc.contributor.authorChodaczek, Grzegorz
dc.contributor.authorJaśpińska, Joanna
dc.contributor.authorPawlikowska-Bartosz, Anna
dc.contributor.authorSłupski, Wojciech
dc.contributor.authorLechniak Dorota
dc.contributor.authorSzumacher, Małgorzata
dc.contributor.authorOlorunlowu, Segun
dc.contributor.authorSzulc, Karolina
dc.contributor.authorCieślak, Adam
dc.contributor.authorŚlusarczyk, Sylwester
dc.date.access2025-03-31
dc.date.accessioned2025-03-31T11:13:01Z
dc.date.available2025-03-31T11:13:01Z
dc.date.copyright2024-12-26
dc.date.issued2025
dc.description.abstract<jats:p>This study aims to explore the effect of pulsed electric field (PEF) treatment as a method very likely to result in reversible electroporation of Scutellaria baicalensis Georgi underground organs, resulting in increased mass transfer and secondary metabolites leakage. PEF treatment with previously established empirically tailored parameters [E = 0.3 kV/cm (U = 3 kV, d = 10 cm), t = 50 µs, N = 33 f = 1 Hz] was applied 1–3 times to S. baicalensis roots submerged in four different Natural Deep Eutectic Solvents (NADES) media (1—choline chloride/xylose (1:2) + 30% water, 2—choline chloride/glucose (1:2) + 30% water, 3—choline chloride/ethylene glycol (1:2), and 4—tap water (EC = 0.7 mS/cm). Confocal microscopy was utilized to visualize the impact of PEF treatment on the root cells in situ. As a result of plant cell membrane permeabilization, an extract containing major active metabolites was successfully acquired in most media, achieving the best results using medium 1 and repeating the PEF treatment twice (baicalein &lt;LOQ, baicalin 12.85 µg/mL, wogonin 2.15 µg/mL, and wogonoside 3.01 µg/mL). Wogonin concentration in NADES media was on par with the control (plants harvested on the day of the experiment, ultrasound-mediated methanolic extraction, Cwogonin = 2.15 µg/mL). After successful extraction, PEF treatment allowed the plants to continue growing, with the lowest survival rate across treated groups being 60%. Additionally, an enhancement in plant growth parameters (length and fresh mass of the roots) and significant changes in the S. baicalensis root phytochemical profile were also observed.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0.0
dc.description.if4,9
dc.description.number1
dc.description.points140
dc.description.versionfinal_published
dc.description.volume26
dc.identifier.doi10.3390/ijms26010100
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2659
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/26/1/100
dc.languageen
dc.pbn.affiliationanimal science and fisheries
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pagesart. 100
dc.relation.projectThe research was funded by: the National Science Centre of Poland grant number UMO-2020/39/D/NZ9/01402 to S.Ś. as well as the Wrocław Medical University grant no. SUBK.D030.24.011, Talenty Jutra grant (1st edition), funded by the Empiria i Wiedza Foundation to Kajetan Grzelka. The Botanical Garden of Medicinal Plants receives support from Polish Ministry of Science and Higher Education for a special research facility, decision No. 28/598769/SPUB/SP/2024.
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.enelectroporation
dc.subject.eneutectic solvents
dc.subject.enflavonoids
dc.subject.enhydroponics
dc.titlePulsed Electric Field (PEF) Treatment Results in Growth Promotion, Main Flavonoids Extraction, and Phytochemical Profile Modulation of Scutellaria baicalensis Georgi Roots
dc.title.volumeSpecial Issue Plant Resilience: Insights into Abiotic and Biotic Stress Adaptations
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume26