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Ex vivo folate production by fecal bacteria does not predict human blood folate status: Associations between dietary patterns, gut microbiota, and folate metabolism

cris.virtual.author-orcid0000-0002-7402-1328
cris.virtual.author-orcid0000-0001-8417-2245
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-2045-0709
cris.virtualsource.author-orcid59851afa-3d5d-435f-95ee-4130cbd454a0
cris.virtualsource.author-orcidedf63522-bb89-4788-a8d3-9c3f3858875b
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid20ac3fe1-f398-4ef7-ab0b-a6a20460f69f
dc.abstract.enAlthough some bacteria inhabiting the human gut synthesize folates, it has not yet been established whether bacterial folate biosynthesis can impact human folate status. The main objectives of this study were to evaluate associations between different lifestyle factors and the potential of fecal microbiota to produce folates, and to investigate whether this potential is associated with circulating folate and total homocysteine (tHcy) levels in humans. To this end, we carried out an observational study of two hundred adult participants, with high variance in dietary habits. Diet was determined using three-day food records. Fecal microbiota composition was assessed by 16S rRNA gene amplicon sequencing. To establish the folate-production potential of fecal bacteria, cultures containing feces were incubated under anaerobic conditions for 24 h, and the folate concentration was measured before and after incubation. The folate concentration in cultures was 185.4 ± 228.1 pg/ml/log(CFU/g) (2125.4 ± 2454.3 pg/ml) higher after incubation. This change in concentration was not associated with the healthy eating index that measures diet quality (r = -0.11, p = 0.11), but it was positively associated with low α-diversity (r = -0.18, p < 0.01), and high relative abundance of the Bacteroides, as well as Sutterella and Parasutterella genera. The gut microbiota’s folate producing potential was associated neither with serum folate nor with plasma tHcy levels. In conclusion, some taxa of the native gut microbiota have the ability to synthesize folates under culture conditions, but this bacterial folate biosynthesis capacity does not predict human folate status.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Żywienia Człowieka i Dietetyki
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.contributor.authorMalinowska, Anna Maria
dc.contributor.authorSchmidt, Marcin
dc.contributor.authorKok, Dieuwertje E.
dc.contributor.authorChmurzyńska, Agata
dc.date.access2026-02-16
dc.date.accessioned2026-02-26T12:33:43Z
dc.date.available2026-02-26T12:33:43Z
dc.date.copyright2022-04-26
dc.date.issued2022
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if8,1
dc.description.numberJune 2022
dc.description.points140
dc.description.versionfinal_published
dc.description.volume156
dc.identifier.doi10.1016/j.foodres.2022.111290
dc.identifier.issn0963-9969
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/7502
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0963996922003477?via%3Dihub
dc.languageen
dc.relation.ispartofFood Research International
dc.relation.pagesart. 111290
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.endietary pattern
dc.subject.engut microbiota
dc.subject.enfolate
dc.subject.enhomocysteine
dc.subject.enbiosynthesis
dc.titleEx vivo folate production by fecal bacteria does not predict human blood folate status: Associations between dietary patterns, gut microbiota, and folate metabolism
dc.typeJournalArticle
dspace.entity.typePublication