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Application of UPLC-MS/MS Method for Analysis of Apigenin, Apigenin 7-Glucoside and Chlorogenic Acid in Goat Serum

cris.virtual.author-orcid0000-0002-9186-006X
cris.virtual.author-orcid0000-0002-9650-7572
cris.virtual.author-orcid0000-0001-7785-2517
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cris.virtualsource.author-orcid6d08bd64-d529-4e9a-a96e-0c48877e79d9
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dc.abstract.enUPLC-MS/MS method was developed for determination of bioactive compounds including chlorogenic acid (CA), apigenin (AP) and apigenin 7-glucoside (AP7G) in goat serum. The analytes were separated on Kinetex C18 analytical column and a mobile phase consisting 0.1% formic acid in water and methanol with gradient elution. Detection of analytes was performed using a triple quadrupole mass spectrometer with electrospray ionization in positive mode. The lower limit of quantitation was 0.5 ng mL–1 for AP and AP7G and 2.5 ng mL–1 for CA. The inter- and intra-day precision of the method expressed as relative standard deviation was in the range of 1.22–14.5%. The accuracy of the method expressed as the relative error was in the range of 0.147–14.6%. The extraction recoveries of the compounds were 76.2–89.5%. The validated method was successfully applied for analysis of the compounds in the serum of sixty goats, half of which were fed with fodder with the addition of an herbal mixture. The higher concentration values of all analyzed compounds were reported in the serum of the animals fed with herbs compared to the control group receiving standard fodder.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Hodowli Zwierząt i Oceny Surowców
dc.affiliation.instituteKatedra Mleczarstwa i Inżynierii Procesowej
dc.contributor.authorKaraźniewicz-Łada, Marta
dc.contributor.authorWójtowski, Jacek
dc.contributor.authorGłówka, Franciszek
dc.contributor.authorDanków-Kubisz, Romualda
dc.contributor.authorPikul, Jan
dc.contributor.authorGryszczyńska, Agnieszka
dc.contributor.authorFoksowicz-Flaczyk, Joanna
dc.contributor.authorMikołajczak, Przemysław Łukasz
dc.date.access2025-06-10
dc.date.accessioned2025-09-19T06:17:53Z
dc.date.available2025-09-19T06:17:53Z
dc.date.copyright2023-04-12
dc.date.issued2023
dc.description.abstract<jats:title>Abstract</jats:title><jats:p>UPLC-MS/MS method was developed for determination of bioactive compounds including chlorogenic acid (CA), apigenin (AP) and apigenin 7-glucoside (AP7G) in goat serum. The analytes were separated on Kinetex C18 analytical column and a mobile phase consisting 0.1% formic acid in water and methanol with gradient elution. Detection of analytes was performed using a triple quadrupole mass spectrometer with electrospray ionization in positive mode. The lower limit of quantitation was 0.5 ng mL<jats:sup>–1</jats:sup> for AP and AP7G and 2.5 ng mL<jats:sup>–1</jats:sup> for CA. The inter- and intra-day precision of the method expressed as relative standard deviation was in the range of 1.22–14.5%. The accuracy of the method expressed as the relative error was in the range of 0.147–14.6%. The extraction recoveries of the compounds were 76.2–89.5%. The validated method was successfully applied for analysis of the compounds in the serum of sixty goats, half of which were fed with fodder with the addition of an herbal mixture. The higher concentration values of all analyzed compounds were reported in the serum of the animals fed with herbs compared to the control group receiving standard fodder.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if1,2
dc.description.number5
dc.description.points70
dc.description.versionfinal_published
dc.description.volume86
dc.identifier.doi10.1007/s10337-023-04250-7
dc.identifier.eissn1612-1112
dc.identifier.issn0009-5893
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4936
dc.identifier.weblinkhttps://link.springer.com/article/10.1007/s10337-023-04250-7
dc.languageen
dc.relation.ispartofChromatographia
dc.relation.pages401-411
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.enbioactive polyphenols
dc.subject.engoats
dc.subject.enMS/MS detection
dc.subject.enprotein precipitation
dc.subject.envalidation
dc.titleApplication of UPLC-MS/MS Method for Analysis of Apigenin, Apigenin 7-Glucoside and Chlorogenic Acid in Goat Serum
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue5
oaire.citation.volume86