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Evolution of CRISPR/Cas Systems for Precise Genome Editing

cris.virtual.author-orcid0000-0002-1496-1502
cris.virtual.author-orcid0000-0002-0199-9245
cris.virtual.author-orcid0000-0002-1208-3291
cris.virtualsource.author-orcida706896c-13b8-4acd-8e84-35ef99d53321
cris.virtualsource.author-orcid5a42524e-79b2-4abc-87a0-36571ec9f87a
cris.virtualsource.author-orcid0e52860c-9c5d-441c-b9ad-689938b2c236
dc.abstract.enThe bacteria-derived CRISPR/Cas (an acronym for regularly interspaced short palindromic repeats/CRISPR-associated protein) system is currently the most widely used, versatile, and convenient tool for genome engineering. CRISPR/Cas-based technologies have been applied to disease modeling, gene therapies, transcriptional modulation, and diagnostics. Nevertheless, some challenges remain, such as the risk of immunological reactions or off-target effects. To overcome these problems, many new methods and CRISPR/Cas-based tools have been developed. In this review, we describe the current classification of CRISPR systems and new precise genome-editing technologies, summarize the latest applications of this technique in several fields of research, and, finally, discuss CRISPR/Cas system limitations, ethical issues, and challenges.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Biochemii i Biotechnologii
dc.contributor.authorHryhorowicz, Magdalena Julita
dc.contributor.authorLipiński, Daniel
dc.contributor.authorZeyland, Joanna
dc.date.access2025-02-25
dc.date.accessioned2025-02-25T12:11:06Z
dc.date.available2025-02-25T12:11:06Z
dc.date.copyright2023-09-18
dc.date.issued2023
dc.description.abstract<jats:p>The bacteria-derived CRISPR/Cas (an acronym for regularly interspaced short palindromic repeats/CRISPR-associated protein) system is currently the most widely used, versatile, and convenient tool for genome engineering. CRISPR/Cas-based technologies have been applied to disease modeling, gene therapies, transcriptional modulation, and diagnostics. Nevertheless, some challenges remain, such as the risk of immunological reactions or off-target effects. To overcome these problems, many new methods and CRISPR/Cas-based tools have been developed. In this review, we describe the current classification of CRISPR systems and new precise genome-editing technologies, summarize the latest applications of this technique in several fields of research, and, finally, discuss CRISPR/Cas system limitations, ethical issues, and challenges.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,9
dc.description.number18
dc.description.points140
dc.description.reviewreview
dc.description.versionfinal_published
dc.description.volume24
dc.identifier.doi10.3390/ijms241814233
dc.identifier.issn1422-0067
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2510
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/24/18/14233
dc.languageen
dc.pbn.affiliationbiotechnology
dc.pbn.affiliationagriculture and horticulture
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pagesart. 14233
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enCRISPR/Cas9 system
dc.subject.enCRISPR classification
dc.subject.enCas9 nuclease
dc.subject.enCas12a nuclease
dc.subject.enCas13a nuclease
dc.subject.engenome editing
dc.subject.enbase editors
dc.subject.enprime editors
dc.titleEvolution of CRISPR/Cas Systems for Precise Genome Editing
dc.title.volumeSpecial Issue CRISPR-Cas Systems and Genome Editing
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue18
oaire.citation.volume24