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Droplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland

cris.virtual.author-orcid0000-0001-6405-8226
cris.virtual.author-orcid0000-0003-2833-5083
cris.virtual.author-orcid0000-0003-3485-1359
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-4238-0414
cris.virtualsource.author-orcid1d618743-2cac-422f-9cd2-db6436511561
cris.virtualsource.author-orcid713c4db4-53f8-4862-ac61-6104db5e840c
cris.virtualsource.author-orcidbc097627-d51d-4472-8ae6-8740c8dadfdf
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid30dfea4c-e71f-4891-8e23-aafe00ca70e0
dc.abstract.enIntroduction MicroRNAs (miRNAs), a class of noncoding small RNAs, have been recognised as potential biomarkers of mammary gland conditions, including bovine mastitis diagnosis. The aim of this study was to quantify selected miRNAs in the milk of mastitic cows. Material and Methods Milk samples (n = 90) were collected from healthy and mastitic dairy cows originating from local dairy cattle farms located in the west of Poland. MicroRNAs of the miR-21a, miR-92a, miR-146a and miR-383 species were quantified using the highly sensitive droplet digital PCR method. Direct measurement of somatic cell count (SCC) was performed using a cell counter. Cows were divided into three groups: those with an SCC below 200,000/mL were designated Low (n = 25), those with an SCC between 200,000 and 999,999 were Medium (n = 34), and those with an SCC of 1,000,000 or higher were High (n = 31). Microbiological analyses were performed using standard culture testing. Results The level of miR-383 was very low and this miRNA was excluded from analysis. The miR-92a was used to normalise miR-21a and miR-146a expression levels. The obtained results of expression of miR-21a and miR-146a correlated with somatic cell number (R = 0.53 and 0.79, respectively). Conclusion These results show that ddPCR is a useful method for quantifying miRNAs in raw cow milk. It seems that miR-146a is a promising marker for bovine mastitis, although further studies are needed to select a panel of miRNAs that can be used in mastitis monitoring in Poland.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Chorób Wewnętrznych i Diagnostyki
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.contributor.authorSmulski, Sebastian
dc.contributor.authorPszczoła, Marcin Jerzy
dc.contributor.authorStachowiak, Monika
dc.contributor.authorBilińska, Adrianna
dc.contributor.authorSzczerbal, Izabela
dc.date.access2025-07-07
dc.date.accessioned2025-09-22T06:07:21Z
dc.date.available2025-09-22T06:07:21Z
dc.date.copyright2023-12-19
dc.date.issued2023
dc.description.abstract<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Introduction</jats:title> <jats:p>MicroRNAs (miRNAs), a class of noncoding small RNAs, have been recognised as potential biomarkers of mammary gland conditions, including bovine mastitis diagnosis. The aim of this study was to quantify selected miRNAs in the milk of mastitic cows.</jats:p> </jats:sec> <jats:sec> <jats:title>Material and Methods</jats:title> <jats:p>Milk samples (n = 90) were collected from healthy and mastitic dairy cows originating from local dairy cattle farms located in the west of Poland. MicroRNAs of the miR-21a, miR-92a, miR-146a and miR-383 species were quantified using the highly sensitive droplet digital PCR method. Direct measurement of somatic cell count (SCC) was performed using a cell counter. Cows were divided into three groups: those with an SCC below 200,000/mL were designated Low (n = 25), those with an SCC between 200,000 and 999,999 were Medium (n = 34), and those with an SCC of 1,000,000 or higher were High (n = 31). Microbiological analyses were performed using standard culture testing.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The level of miR-383 was very low and this miRNA was excluded from analysis. The miR-92a was used to normalise miR-21a and miR-146a expression levels. The obtained results of expression of miR-21a and miR-146a correlated with somatic cell number (R = 0.53 and 0.79, respectively).</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>These results show that ddPCR is a useful method for quantifying miRNAs in raw cow milk. It seems that miR-146a is a promising marker for bovine mastitis, although further studies are needed to select a panel of miRNAs that can be used in mastitis monitoring in Poland.</jats:p> </jats:sec>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if1,3
dc.description.number4
dc.description.points200
dc.description.versionfinal_published
dc.description.volume67
dc.identifier.doi10.2478/jvetres-2023-0062
dc.identifier.eissn2450-8608
dc.identifier.issn2450-7393
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4996
dc.identifier.weblinkhttps://sciendo.com/pl/article/10.2478/jvetres-2023-0062
dc.languageen
dc.relation.ispartofJournal of Veterinary Research (Poland)
dc.relation.pages583-591
dc.rightsCC-BY-NC-ND
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.enbiomarker
dc.subject.enbovine mastitis
dc.subject.enddPCR
dc.subject.enmicroRNA
dc.subject.enraw milk.
dc.titleDroplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue4
oaire.citation.volume67