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Droplet Digital PCR Quantification of Selected Intracellular and Extracellular microRNAs Reveals Changes in Their Expression Pattern during Porcine In Vitro Adipogenesis

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cris.virtual.author-orcid0000-0003-2833-5083
cris.virtual.author-orcid0000-0003-3485-1359
cris.virtual.author-orcid0000-0003-3635-7274
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cris.virtual.author-orcid0000-0002-4238-0414
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cris.virtualsource.author-orcid713c4db4-53f8-4862-ac61-6104db5e840c
cris.virtualsource.author-orcidbc097627-d51d-4472-8ae6-8740c8dadfdf
cris.virtualsource.author-orcid493060ba-4c3c-41cc-8560-0bee347b7418
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dc.abstract.enExtracellular miRNAs have attracted considerable interest because of their role in intercellular communication, as well as because of their potential use as diagnostic and prognostic biomarkers for many diseases. It has been shown that miRNAs secreted by adipose tissue can contribute to the pathophysiology of obesity. Detailed knowledge of the expression of intracellular and extracellular microRNAs in adipocytes is thus urgently required. The system of in vitro differentiation of mesenchymal stem cells (MSCs) into adipocytes offers a good model for such an analysis. The aim of this study was to quantify eight intracellular and extracellular miRNAs (miR-21a, miR-26b, miR-30a, miR-92a, miR-146a, miR-148a, miR-199, and miR-383a) during porcine in vitro adipogenesis using droplet digital PCR (ddPCR), a highly sensitive method. It was found that only some miRNAs associated with the inflammatory process (miR-21a, miR-92a) were highly expressed in differentiated adipocytes and were also secreted by cells. All miRNAs associated with adipocyte differentiation were highly abundant in both the studied cells and in the cell culture medium. Those miRNAs showed a characteristic expression profile with upregulation during differentiation.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.contributor.authorBilinska, Adrianna
dc.contributor.authorPszczoła, Marcin Jerzy
dc.contributor.authorStachowiak, Monika
dc.contributor.authorDługosz (zd. Stachecka), Joanna Magdalena
dc.contributor.authorGarbacz, Franciszek
dc.contributor.authorAksoy, Mehmet Onur
dc.contributor.authorSzczerbal, Izabela
dc.date.access2025-06-24
dc.date.accessioned2025-09-10T08:01:22Z
dc.date.available2025-09-10T08:01:22Z
dc.date.copyright2023-03-09
dc.date.issued2023
dc.description.abstract<jats:p>Extracellular miRNAs have attracted considerable interest because of their role in intercellular communication, as well as because of their potential use as diagnostic and prognostic biomarkers for many diseases. It has been shown that miRNAs secreted by adipose tissue can contribute to the pathophysiology of obesity. Detailed knowledge of the expression of intracellular and extracellular microRNAs in adipocytes is thus urgently required. The system of in vitro differentiation of mesenchymal stem cells (MSCs) into adipocytes offers a good model for such an analysis. The aim of this study was to quantify eight intracellular and extracellular miRNAs (miR-21a, miR-26b, miR-30a, miR-92a, miR-146a, miR-148a, miR-199, and miR-383a) during porcine in vitro adipogenesis using droplet digital PCR (ddPCR), a highly sensitive method. It was found that only some miRNAs associated with the inflammatory process (miR-21a, miR-92a) were highly expressed in differentiated adipocytes and were also secreted by cells. All miRNAs associated with adipocyte differentiation were highly abundant in both the studied cells and in the cell culture medium. Those miRNAs showed a characteristic expression profile with upregulation during differentiation.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,8
dc.description.number3
dc.description.points100
dc.description.versionfinal_published
dc.description.volume14
dc.identifier.doi10.3390/genes14030683
dc.identifier.issn2073-4425
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4703
dc.identifier.weblinkhttps://www.mdpi.com/2073-4425/14/3/683
dc.languageen
dc.relation.ispartofGenes
dc.relation.pagesart. 683
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.enadipocytes
dc.subject.enddPCR
dc.subject.enECmiRNAs
dc.subject.enmesenchymal stem cells
dc.subject.enpig
dc.titleDroplet Digital PCR Quantification of Selected Intracellular and Extracellular microRNAs Reveals Changes in Their Expression Pattern during Porcine In Vitro Adipogenesis
dc.title.volumeSpecial Issue Trends and Prospects in Pig Genomics and Genetics
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue3
oaire.citation.volume14