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Quantitative Analysis of Lactobionic Acid in Bioreactor Cultures and Selected Biological Activities

cris.virtual.author-orcid0000-0003-4724-075X
cris.virtual.author-orcid0000-0002-9477-8563
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid23456f91-6f69-419b-9fb9-612ef544e6ad
cris.virtualsource.author-orcid73a2e591-ad92-47ae-b1e4-3eb06ca9d58f
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enThe aim of this study was to quantitatively analyse lactobionic acid obtained from bioreactor cultures using whey as a liquid medium with bacteria of the Pseudomonas taetrolens species. The most important culture parameters affecting the production of the acid are indicated and evaluated. The highest lactobionic acid yield was 37.42 g/L, selecting the appropriate strain (Pseudomonas taetrolens 4′) and environmental conditions (2% lactose concentration in the bioreactor). The amount of lactose and lactobionic acid was determined by high-performance liquid chromatography. Microorganism analysis was also carried out using a flow cytometer with imaging to study the metabolic activity of microorganisms during lactobionic acid production. In addition, during the study, Bifidobacteria were microencapsulated with lactobionic acid and their survival was evaluated in an in vitro model of the gastrointestinal tract, checking the prebiotic properties of the acid. The highest number of viable cells in the microcapsules after digestion was obtained using the Bifidobacterium bifidum strain DSM 20082. The antagonistic activity of lactobionic acid was also analysed.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Technologii Żywności Pochodzenia Roślinnego
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.contributor.authorGoderska, Kamila
dc.contributor.authorJuzwa, Wojciech
dc.contributor.authorKarpiński, Tomasz M.
dc.date.access2024-12-02
dc.date.accessioned2024-12-10T08:40:58Z
dc.date.available2024-12-10T08:40:58Z
dc.date.copyright2024-11-15
dc.date.issued2024
dc.description.abstract<jats:p>The aim of this study was to quantitatively analyse lactobionic acid obtained from bioreactor cultures using whey as a liquid medium with bacteria of the Pseudomonas taetrolens species. The most important culture parameters affecting the production of the acid are indicated and evaluated. The highest lactobionic acid yield was 37.42 g/L, selecting the appropriate strain (Pseudomonas taetrolens 4′) and environmental conditions (2% lactose concentration in the bioreactor). The amount of lactose and lactobionic acid was determined by high-performance liquid chromatography. Microorganism analysis was also carried out using a flow cytometer with imaging to study the metabolic activity of microorganisms during lactobionic acid production. In addition, during the study, Bifidobacteria were microencapsulated with lactobionic acid and their survival was evaluated in an in vitro model of the gastrointestinal tract, checking the prebiotic properties of the acid. The highest number of viable cells in the microcapsules after digestion was obtained using the Bifidobacterium bifidum strain DSM 20082. The antagonistic activity of lactobionic acid was also analysed.</jats:p>
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,2
dc.description.number22
dc.description.points140
dc.description.volume29
dc.identifier.doi10.3390/molecules29225400
dc.identifier.issn1420-3049
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2190
dc.identifier.weblinkhttps://www.mdpi.com/1420-3049/29/22/5400
dc.languageen
dc.relation.ispartofMolecules
dc.relation.pagesart. 5400
dc.rightsCC-BY
dc.sciencecloudsend
dc.subject.enlactobionic acid
dc.subject.enPseudomonas taetrolens
dc.subject.enwhey
dc.subject.enlactose
dc.subject.enflow cytometry
dc.subject.enBifidobacterium
dc.subject.enprebiotic
dc.subject.enin vitro model
dc.titleQuantitative Analysis of Lactobionic Acid in Bioreactor Cultures and Selected Biological Activities
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue22
oaire.citation.volume29