Analysis of miRNA expression associated with gene Lr34 responsible for resistance mechanisms to wheat leaf rust
Type
Journal article
Language
English
Date issued
2023
Author
Spychala, Julia
Kuczynski, Jakub
Tyczewska, Agata
Faculty
Wydział Rolnictwa, Ogrodnictwa i Biotechnologii
Wydział Nauk o Żywności i Żywieniu
Journal
Pakistan Journal of Botany
ISSN
0556-3321
Volume
55
Number
1
Pages from-to
379-385
Abstract (EN)
Diseases caused by pathogenic fungi significantly limit the yield and quality of common wheat. The most dangerous
fungal diseases of wheat include leaf rust, stripe rust and powdery mildew. Cultivating resistant wheat varieties is the most
effective way to minimize the development of fungal diseases. Genetically determined resistance of the horizontal type
(racially non-specific, adult plant resistance genes) is preferred due to its more persistent expression compared to the major
(R) genes determining race-specific resistance, which is often overcome by pathogens. Currently, hexaploid wheat
resistance gene Lr34/Yr18/Sr57/Pm38 (hereafter referred to as Lr34) is the best characterized gene determining horizontaltype resistance. The aim of this study was to analyze miRNA expression in selected common wheat varieties, showing the
presence of resistance gene Lr34 in response to infection with the fungus Puccinia triticina responsible for leaf rust. The
reference varieties (Pavon'S', Myna'S', Frontana'S' and Sparrow'S') contained slow rust resistance genes, including Lr34,
whereas the control variant HN ROD did not have these genes. The presence of Lr34 in four reference varieties was
confirmed using polymerase chain reaction (PCR). Biotic stress in adult plants was induced by inoculating fungal spores
under monitored conditions in a growth chamber. Differences in the expression of various microRNAs (miR9653b,
miR9657b, miR9773, miR9677b) associated with gene Lr34 were tested using emulsion PCR (ddPCR). Plant material for
analysis was collected before inoculation and 6, 12, 24 and 48 hours after inoculation. The results showed an increase in
miR9653b expression in varieties carrying gene Lr34 as a result of plant infection with P. triticina. The ddPCR analysis of
miR9657b, miR9773 and miR9677b yielded too low a copy number for correct inference. The expression level of
miR9653b in a control variety (HN ROD) lacking this resistance gene remained stable. This demonstrated that miR9653b
could be involved in plant resistance mechanisms in response to leaf rust.
fungal diseases of wheat include leaf rust, stripe rust and powdery mildew. Cultivating resistant wheat varieties is the most
effective way to minimize the development of fungal diseases. Genetically determined resistance of the horizontal type
(racially non-specific, adult plant resistance genes) is preferred due to its more persistent expression compared to the major
(R) genes determining race-specific resistance, which is often overcome by pathogens. Currently, hexaploid wheat
resistance gene Lr34/Yr18/Sr57/Pm38 (hereafter referred to as Lr34) is the best characterized gene determining horizontaltype resistance. The aim of this study was to analyze miRNA expression in selected common wheat varieties, showing the
presence of resistance gene Lr34 in response to infection with the fungus Puccinia triticina responsible for leaf rust. The
reference varieties (Pavon'S', Myna'S', Frontana'S' and Sparrow'S') contained slow rust resistance genes, including Lr34,
whereas the control variant HN ROD did not have these genes. The presence of Lr34 in four reference varieties was
confirmed using polymerase chain reaction (PCR). Biotic stress in adult plants was induced by inoculating fungal spores
under monitored conditions in a growth chamber. Differences in the expression of various microRNAs (miR9653b,
miR9657b, miR9773, miR9677b) associated with gene Lr34 were tested using emulsion PCR (ddPCR). Plant material for
analysis was collected before inoculation and 6, 12, 24 and 48 hours after inoculation. The results showed an increase in
miR9653b expression in varieties carrying gene Lr34 as a result of plant infection with P. triticina. The ddPCR analysis of
miR9657b, miR9773 and miR9677b yielded too low a copy number for correct inference. The expression level of
miR9653b in a control variety (HN ROD) lacking this resistance gene remained stable. This demonstrated that miR9653b
could be involved in plant resistance mechanisms in response to leaf rust.
License
Other
Other Open access date
February 2023