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Identification and Analysis of Candidate Genes Associated with Maize Fusarium Cob Resistance Using Next-Generation Sequencing Technology

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cris.virtual.author-orcid0000-0001-9516-8911
cris.virtual.author-orcid0000-0002-0102-0084
cris.virtual.author-orcid0000-0003-2358-9068
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cris.virtualsource.author-orcid2faa4bbb-a129-4bec-8137-bd8eeedf40e0
cris.virtualsource.author-orcid51a5a68b-106b-4e9d-bd9b-79d15d3ec0c1
cris.virtualsource.author-orcidd32ae288-443c-48d5-a13c-74de2fe7037f
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dc.abstract.enThe pressure to reduce mineral fertilization and the amount of pesticides used has become a factor limiting production growth, as has the elimination of many crop protection chemicals from the market. A key condition for this to be an effective form of protection is the use of varieties with higher levels of resistance. The most effective and fastest way to assist in the selection and control of pathogens is the conducting of genome-wide association studies. These are useful tools for identifying candidate genes, especially when combined with QTL mapping to map and validate loci for quantitative traits. The aim of this study was to identify new markers coupled to genes that determine maize plant resistance to fusarium head blight through the use of next-generation sequencing, association and physical mapping, and to optimize diagnostic procedures to identify selected molecular markers coupled to plant resistance to this fungal disease. As a result of field experiments and molecular analyses, molecular markers coupled to potential genes for resistance to maize ear fusariosis were selected. The newly selected markers were tested against reference genotypes. As a result of the analyses, it was found that two markers (11801 and 20607) out of the ten that were tested differentiated between susceptible and resistant genotypes. Marker number 11801 proved to be the most effective, with a specious product of 237 bp appearing for genotypes 1, 3, 5, 9 and 10. These genotypes were characterized by a field resistance of 4–6 on the 9° scale (1 being susceptible, 9 being resistant) and for all genotypes except 16 and 20, which were characterized by a field resistance of 9. In the next step, this marker will be tested on a wider population of extreme genotypes in order to use it for the preliminary selection of fusarium-resistant genotypes, and the phosphoenolpyruvate carboxylase kinase 1 gene coupled to it will be subjected to expression analysis.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Metod Matematycznych i Statystycznych
dc.affiliation.instituteKatedra Genetyki i Hodowli Roślin
dc.affiliation.instituteKatedra Agronomii
dc.contributor.authorSobiech, Aleksandra
dc.contributor.authorTomkowiak, Agnieszka
dc.contributor.authorBocianowski, Jan
dc.contributor.authorSzymańska, Grażyna
dc.contributor.authorNowak, Bartosz
dc.contributor.authorLenort, Maciej
dc.date.access2025-09-01
dc.date.accessioned2025-09-01T07:26:19Z
dc.date.available2025-09-01T07:26:19Z
dc.date.copyright2023-11-24
dc.date.issued2023
dc.description.abstract<jats:p>The pressure to reduce mineral fertilization and the amount of pesticides used has become a factor limiting production growth, as has the elimination of many crop protection chemicals from the market. A key condition for this to be an effective form of protection is the use of varieties with higher levels of resistance. The most effective and fastest way to assist in the selection and control of pathogens is the conducting of genome-wide association studies. These are useful tools for identifying candidate genes, especially when combined with QTL mapping to map and validate loci for quantitative traits. The aim of this study was to identify new markers coupled to genes that determine maize plant resistance to fusarium head blight through the use of next-generation sequencing, association and physical mapping, and to optimize diagnostic procedures to identify selected molecular markers coupled to plant resistance to this fungal disease. As a result of field experiments and molecular analyses, molecular markers coupled to potential genes for resistance to maize ear fusariosis were selected. The newly selected markers were tested against reference genotypes. As a result of the analyses, it was found that two markers (11801 and 20607) out of the ten that were tested differentiated between susceptible and resistant genotypes. Marker number 11801 proved to be the most effective, with a specious product of 237 bp appearing for genotypes 1, 3, 5, 9 and 10. These genotypes were characterized by a field resistance of 4–6 on the 9° scale (1 being susceptible, 9 being resistant) and for all genotypes except 16 and 20, which were characterized by a field resistance of 9. In the next step, this marker will be tested on a wider population of extreme genotypes in order to use it for the preliminary selection of fusarium-resistant genotypes, and the phosphoenolpyruvate carboxylase kinase 1 gene coupled to it will be subjected to expression analysis.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_research
dc.description.financecost11176,89
dc.description.if4,9
dc.description.number23
dc.description.points140
dc.description.versionfinal_published
dc.description.volume24
dc.identifier.doi10.3390/ijms242316712
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4546
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/24/23/16712
dc.languageen
dc.pbn.affiliationagriculture and horticulture
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pagesart. 16712
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.enmaize
dc.subject.enNGS
dc.subject.enassociation mapping
dc.subject.enfusarium
dc.subject.enSNP markers
dc.subject.enSilicoDArT markers
dc.titleIdentification and Analysis of Candidate Genes Associated with Maize Fusarium Cob Resistance Using Next-Generation Sequencing Technology
dc.title.volumeSpecial Issue Molecular Genetics and Plant Breeding 3.0
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue23
oaire.citation.volume24