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Dynamics of nitration during dark-induced leaf senescence in Arabidopsis reveals proteins modified by tryptophan nitration

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cris.virtual.author-orcid0000-0003-2281-223X
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cris.virtualsource.author-orcid4e2dc392-8cf9-4b89-a596-333bcc45d7cd
dc.abstract.enNitric oxide (NO) is a critical molecule that links plant development with stress responses. Herein, new insights into the role of NO metabolism during leaf senescence in Arabidopsis are presented. A gradual decrease in NO emission accompanied dark-induced leaf senescence (DILS), and a transient wave of peroxynitrite (ONOO–) formation was detected by day 3 of DILS. The boosted ONOO– did not promote tryptophan (Trp) nitration, while the pool of 6-nitroTrp-containing proteins was depleted as senescence progressed. Immunoprecipitation combined with mass spectrometry was used to identify 63 and 4 characteristic 6-nitroTrp-containing proteins in control and individually darkened leaves, respectively. The potential in vivo targets of Trp nitration were mainly related to protein biosynthesis and carbohydrate metabolism. In contrast, nitration of tyrosine-containing proteins was intensified 2-fold on day 3 of DILS. Also, nitrative modification of RNA and DNA increased significantly on days 3 and 7 of DILS, respectively. Taken together, ONOO– can be considered a novel pro-senescence regulator that fine-tunes the redox environment for selective bio-target nitration. Thus, DILS-triggered nitrative changes at RNA and protein levels promote developmental shifts during the plant’s lifespan and temporal adjustment in plant metabolism under suboptimal environmental conditions.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Bioinżynierii
dc.affiliation.instituteKatedra Fizjologii Roślin
dc.contributor.authorArasimowicz-Jelonek, Magdalena
dc.contributor.authorJagodzik, Przemysław
dc.contributor.authorPłóciennik, Artur
dc.contributor.authorSobieszczuk-Nowicka, Ewa
dc.contributor.authorMattoo, Autar
dc.contributor.authorPolcyn, Władysław
dc.contributor.authorFloryszak-Wieczorek, Jolanta
dc.date.accessioned2026-03-05T11:07:35Z
dc.date.available2026-03-05T11:07:35Z
dc.date.issued2022
dc.description.abstract<jats:title>Abstract</jats:title> <jats:p>Nitric oxide (NO) is a critical molecule that links plant development with stress responses. Herein, new insights into the role of NO metabolism during leaf senescence in Arabidopsis are presented. A gradual decrease in NO emission accompanied dark-induced leaf senescence (DILS), and a transient wave of peroxynitrite (ONOO–) formation was detected by day 3 of DILS. The boosted ONOO– did not promote tryptophan (Trp) nitration, while the pool of 6-nitroTrp-containing proteins was depleted as senescence progressed. Immunoprecipitation combined with mass spectrometry was used to identify 63 and 4 characteristic 6-nitroTrp-containing proteins in control and individually darkened leaves, respectively. The potential in vivo targets of Trp nitration were mainly related to protein biosynthesis and carbohydrate metabolism. In contrast, nitration of tyrosine-containing proteins was intensified 2-fold on day 3 of DILS. Also, nitrative modification of RNA and DNA increased significantly on days 3 and 7 of DILS, respectively. Taken together, ONOO– can be considered a novel pro-senescence regulator that fine-tunes the redox environment for selective bio-target nitration. Thus, DILS-triggered nitrative changes at RNA and protein levels promote developmental shifts during the plant’s lifespan and temporal adjustment in plant metabolism under suboptimal environmental conditions.</jats:p>
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if6,9
dc.description.number19
dc.description.points140
dc.description.volume73
dc.identifier.doi10.1093/jxb/erac341
dc.identifier.eissn1460-2431
dc.identifier.issn0022-0957
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/7627
dc.languageen
dc.relation.ispartofJournal of Experimental Botany
dc.relation.pages6853-6875
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enarabidopsis
dc.subject.endark-induced leaf senescence
dc.subject.ennitration
dc.subject.ennitric oxide
dc.subject.ennitrotryptophan
dc.subject.ennitrotyrosine
dc.subject.enperoxynitrite
dc.titleDynamics of nitration during dark-induced leaf senescence in Arabidopsis reveals proteins modified by tryptophan nitration
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue19
oaire.citation.volume73