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Simultaneous medium chain carboxylic acids and 1,3-propanediol production in a bioaugmented lactate-based chain elongation induced with glycerol

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cris.virtual.author-orcid0000-0002-9477-8563
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cris.virtualsource.author-orcid73a2e591-ad92-47ae-b1e4-3eb06ca9d58f
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dc.abstract.enThe objective was to investigate the impact of the bioaugmentation on chain elongation process using glycerol, lactate and lactose as substrates in an open culture fermentation. In the batch trials the highest selectivity for chain elongation product, i.e. caproate, was observed in trials inoculated with co-culture of Megasphaera elsdenii and Eubacterium limosum grown on glycerol (28.6%), and in non-bioaugmented open culture run on lactose + lactate (14.8%). The results showed that E. limosum, out of two bioaugmented strains, was able to survive in the open culture. A continuous open culture fermentation of glycerol led to caproate and 1,3-propanediol (1,3-PDO) formation, while lactate addition led to 1,3-PDO and short chain carboxylates production. Moving the process into batch mode triggered even-carbon chain elongation. Presence of E. limosum promoted odd-carbon chain elongation and valerate production. Imaging flow cytometry combined with machine learning enabled the discrimination of Eubacterium cells from other microbial strains during the process.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.contributor.authorDuber, Anna
dc.contributor.authorZagrodnik, Roman
dc.contributor.authorJuzwa, Wojciech
dc.contributor.authorGutowska, Natalia
dc.contributor.authorOleskowicz-Popiel, Piotr
dc.date.access2025-06-06
dc.date.accessioned2025-08-12T09:31:02Z
dc.date.available2025-08-12T09:31:02Z
dc.date.copyright2023-12-01
dc.date.issued2024
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if9,0
dc.description.numberFebruary 2024
dc.description.points140
dc.description.versionfinal_published
dc.description.volume393
dc.identifier.doi10.1016/j.biortech.2023.130123
dc.identifier.eissn1873-2976
dc.identifier.issn0960-8524
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4149
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0960852423015511
dc.languageen
dc.relation.ispartofBioresource Technology
dc.relation.pagesart. 130123
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.enmixed culture fermentation
dc.subject.enbioaugmentation
dc.subject.enlactic acid
dc.subject.envaleric acid
dc.subject.encaproic acid
dc.subject.enEubacterium limosum
dc.subject.enimaging flow cytometry
dc.subject.enmachine learning
dc.titleSimultaneous medium chain carboxylic acids and 1,3-propanediol production in a bioaugmented lactate-based chain elongation induced with glycerol
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume393