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Comparison of Dip‐it‐DART‐Orbitrap‐MS With Nitrogen Plasma to HPLC/Orbitrap‐MS in Profiling Aromatic Glycoconjugation in White Grapes

cris.virtual.author-orcid0000-0002-6568-8155
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid1ddb8111-48d2-4a19-a7fb-97562fc1691c
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enDirect analysis of aromatic glycosidic precursors in plants has posed an analytical challenge for decades. Traditional techniques, such as SPE-GC/MS, primarily provided information on volatile aglycones released through hydrolysis. However, the application of high-resolution mass spectrometry combined with liquid chromatography has enabled the direct analysis of intact glycosides without the need for derivatization or hydrolysis. Advances in soft ionization methods, such as DART, offer a novel approach to exploring the hidden aromatic potential in grapes without chromatographic separation. In this work, we present a novel and rapid method for screening aromatic glycosidic precursors in white grapes using high-resolution mass spectrometry (Orbitrap) combined with the soft ionization DART method with nitrogen plasma. Optimization of N2-DART ionization parameters, including grid voltage, gas temperature, and Dip-it sampler speed, performed on selected synthetic glycosidic precursors, allowed the establishment of characteristic ionization patterns and evaluation of 15 groups of glycosidic precursors. The results from the profiling analysis using the N2-DART-Orbitrap-MS method are comparable to those obtained by HPLC/Orbitrap-MS method. This new analytical approach, N2-DART-Orbitrap-MS, reduces drastically analysis time by eliminating the need for chromatographic separation when screening glycoside precursors, uses a convenient Dip-it tips for sampling. It also allows for deeper exploration of ionization using nitrogen plasma, applied for the first time in the analysis of glycoside precursors, demonstrating the applicability of this method for the rapid characterization and screening of glycosidically bound aroma compounds in plants.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Technologii Żywności Pochodzenia Roślinnego
dc.contributor.authorDziadas, Mariusz
dc.contributor.authorJeleń, Henryk
dc.date.accessioned2025-10-23T10:28:43Z
dc.date.available2025-10-23T10:28:43Z
dc.date.issued2025
dc.description.abstract<jats:title>ABSTRACT</jats:title><jats:p>Direct analysis of aromatic glycosidic precursors in plants has posed an analytical challenge for decades. Traditional techniques, such as SPE‐GC/MS, primarily provided information on volatile aglycones released through hydrolysis. However, the application of high‐resolution mass spectrometry combined with liquid chromatography has enabled the direct analysis of intact glycosides without the need for derivatization or hydrolysis. Advances in soft ionization methods, such as DART, offer a novel approach to exploring the hidden aromatic potential in grapes without chromatographic separation. In this work, we present a novel and rapid method for screening aromatic glycosidic precursors in white grapes using high‐resolution mass spectrometry (Orbitrap) combined with the soft ionization DART method with nitrogen plasma. Optimization of N<jats:sub>2</jats:sub>‐DART ionization parameters, including grid voltage, gas temperature, and Dip‐it sampler speed, performed on selected synthetic glycosidic precursors, allowed the establishment of characteristic ionization patterns and evaluation of 15 groups of glycosidic precursors. The results from the profiling analysis using the N<jats:sub>2</jats:sub>‐DART‐Orbitrap‐MS method are comparable to those obtained by HPLC/Orbitrap‐MS method. This new analytical approach, N<jats:sub>2</jats:sub>‐DART‐Orbitrap‐MS, reduces drastically analysis time by eliminating the need for chromatographic separation when screening glycoside precursors, uses a convenient Dip‐it tips for sampling. It also allows for deeper exploration of ionization using nitrogen plasma, applied for the first time in the analysis of glycoside precursors, demonstrating the applicability of this method for the rapid characterization and screening of glycosidically bound aroma compounds in plants.</jats:p>
dc.description.additionalThis work was supported by Narodowe Centrum Nauki (2013/09/D/NZ9/03115).
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,0
dc.description.number5
dc.description.points70
dc.description.volume60
dc.identifier.doi10.1002/jms.5130
dc.identifier.eissn1096-9888
dc.identifier.issn1076-5174
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5412
dc.languageen
dc.relation.ispartofJournal of Mass Spectrometry
dc.relation.pagese5130
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enDART
dc.subject.englycoside precursors
dc.subject.enmonoterpenes
dc.subject.enOrbitrap
dc.subject.enPenning ionization
dc.titleComparison of Dip‐it‐DART‐Orbitrap‐MS With Nitrogen Plasma to HPLC/Orbitrap‐MS in Profiling Aromatic Glycoconjugation in White Grapes
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue5
oaire.citation.volume60