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A new nuclear marker for quantitative analysis of wild boar and domestic pig meat in game meat products using PLAG1 zinc finger gene

cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-6331-5726
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid096e41a9-6ac3-4aa4-9495-6e2699f7f3a3
cris.virtualsource.author-orcid6b8ff0a0-7556-4635-ae2c-064721f8c43a
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enWild boar meat and pork are commonly added to game products, either as forefront or background ingredients. Due to their lower cost and wider availability compared to deer species, there is a potential for producers to misrepresent the inclusion of these species in more expensive game products. Therefore, a novel, highly specific TaqMan qPCR method targeting a single-copy 127-bp region of the PLAG1 zinc finger gene was developed for quantifying these subspecies in processed foodstuff. The individual qPCR systems, adapted to different processing conditions (raw and thermally treated), were implemented using a matrix-adapted reference system. These systems were validated using laboratory-prepared wild boar-in-beef patties. The assay achieved satisfactory performance parameters for fraud detection, reaching a sensitivity of 0.1 ng of wild boar or pig DNA, and both detection and quantification limits (LOD and LOQ) at 0.1% for raw and 0.5% (w/w) for thermally processed samples, respectively. Subsequently, commercial game meat and pork products were analyzed to assess their compliance with labeling claims. The results revealed instances of undeclared species, likely intentional mislabeling, and cross-contamination. The developed method can serve as a valuable tool for ensuring label compliance, preventing food fraud, and upholding halal specifications in the game meat industry.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Technologii Mięsa
dc.contributor.authorAdenuga, Bukola M.
dc.contributor.authorSpychaj, Anita
dc.contributor.authorMontowska, Magdalena
dc.date.access2025-10-24
dc.date.accessioned2025-10-24T06:36:50Z
dc.date.available2025-10-24T06:36:50Z
dc.date.copyright2025-07-01
dc.date.issued2025
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_research
dc.description.financecost12853,59
dc.description.if3,9
dc.description.points140
dc.description.versionfinal_published
dc.description.volume15
dc.identifier.doi10.1038/s41598-025-05167-x
dc.identifier.issn2045-2322
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5431
dc.identifier.weblinkhttps://www.nature.com/articles/s41598-025-05167-x
dc.languageen
dc.pbn.affiliationfood and nutrition technology
dc.relation.ispartofScientific Reports
dc.relation.pagesart. 20454
dc.relation.projectPorównawcza peptydomiczna i genetyczna analiza autentyczności żywności pochodzenia zwierzęcego
dc.rightsCC-BY-NC-ND
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enfood authentication
dc.subject.enmeat products
dc.subject.enwild boar/pork quantification
dc.subject.ennuclear marker
dc.subject.enqPCR
dc.titleA new nuclear marker for quantitative analysis of wild boar and domestic pig meat in game meat products using PLAG1 zinc finger gene
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume15
project.funder.nameNarodowe Centrum Nauki, projekt 2020/37/B/NZ9/00082.