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Betaine supplementation modulates betaine concentration by methylenetetrahydrofolate reductase genotype, but has no effect on amino acid profile in healthy active males: A randomized placebo-controlled cross-over study

cris.virtual.author-orcid0000-0002-9030-1805
cris.virtual.author-orcid0000-0001-5324-5982
cris.virtual.author-orcid0000-0002-6568-8155
cris.virtual.author-orcid0000-0003-4570-7221
cris.virtual.author-orcid0000-0002-5041-2981
cris.virtual.author-orcid0000-0002-2045-0709
cris.virtualsource.author-orcidd9cad178-0d31-4dd0-a418-60d6ed7cfa5d
cris.virtualsource.author-orcidad6302d1-4126-47ee-bfde-d3636e0b6d0a
cris.virtualsource.author-orcid1ddb8111-48d2-4a19-a7fb-97562fc1691c
cris.virtualsource.author-orciddafe00c4-99bb-45f5-8886-d6f261ff6cb3
cris.virtualsource.author-orcid27a945b5-0dd6-465b-9459-95b0fa8038ea
cris.virtualsource.author-orcid20ac3fe1-f398-4ef7-ab0b-a6a20460f69f
dc.abstract.enBetaine supplementation is used by athletes, but its mechanism of action is still not fully understood. We hypothesized that betaine supplementation would increase betaine concentration and alter amino acid profiles in relation to MTHFR genotype and dose in physically active males. The study followed a randomized placebo-controlled cross-over design. Blood samples were collected before and after each supplementation period. Serum was analyzed for amino acid profile, homocysteine, betaine, choline, and trimethylamine N-oxide (TMAO) concentrations. For the washout analysis, only participants starting with betaine were included (n = 20). Statistical analysis revealed no differences in the amino acid profile after betaine supplementation. However, betaine concentration significantly increased after betaine supplementation (from 4.89 ± 1.59 µg/mL to 17.31 ± 9.21 µg/mL, P < .001), with a greater increase observed in MTHFR (C677T, rs180113) T-allele carriers compared to CC (P = .027). Betaine supplementation caused a decrease in homocysteine concentration (from 17.04 ± 4.13 µmol/L to 15.44 ± 3.48 µmol/L, P = .00005) and a non-significant increase in TMAO concentrations (from 0.27 ± 0.20 µg/ml to 0.44 ± 0.70 µg/ml, P = .053), but had no effect on choline concentrations. Serum betaine concentrations were not significantly different after the 21-day washout from the baseline values (baseline: 4.93 ± 1.87 µg/mL and after washout: 4.70 ± 1.70 µg/mL, P = 1.000). In conclusion, betaine supplementation increased betaine and decreased homocysteine concentrations, but did not affect the amino acid profile or choline concentrations in healthy active males. Betaine concentrations may be dependent on MTHFR genotype.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Żywienia Człowieka i Dietetyki
dc.affiliation.instituteKatedra Technologii Żywności Pochodzenia Roślinnego
dc.contributor.authorZawieja, Emilia
dc.contributor.authorDrabińska, Natalia
dc.contributor.authorJeleń, Henryk
dc.contributor.authorSzwengiel, Artur
dc.contributor.authorDurkalec-Michalski, Krzysztof
dc.contributor.authorChmurzyńska, Agata
dc.date.accessioned2024-08-14T09:06:58Z
dc.date.available2024-08-14T09:06:58Z
dc.date.issued2024
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3,4
dc.description.numberJuly
dc.description.points70
dc.description.reviewreview
dc.description.volume127
dc.identifier.doi10.1016/j.nutres.2024.05.003
dc.identifier.issn0271-5317
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/1648
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0271531724000691
dc.languageen
dc.pbn.affiliationfood and nutrition technology
dc.relation.ispartofNutrition Research
dc.relation.pages63-74
dc.rightsClosedAccess
dc.sciencecloudsend
dc.subject.enone-carbon metabolism
dc.subject.enamino acids
dc.subject.enbetaine
dc.subject.entrimethylamine N-oxie
dc.subject.enMTHFR
dc.titleBetaine supplementation modulates betaine concentration by methylenetetrahydrofolate reductase genotype, but has no effect on amino acid profile in healthy active males: A randomized placebo-controlled cross-over study
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume127
project.funder.nameb.d.