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Identification and Potential Participation of Lipases in Autophagic Body Degradation in Embryonic Axes of Lupin (Lupinus spp.) Germinating Seeds

cris.virtual.author-orcid0000-0001-9875-3112
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cris.virtualsource.author-orcidf23de9f1-7797-4798-8362-6d5d20dccdef
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cris.virtualsource.author-orcid8f92b7c4-8d55-46a7-a433-009335e22382
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cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enAutophagy is a fundamental process for plants that plays a crucial role in maintainingcellular homeostasis and promoting survival in response to various environmental stresses. One ofthe lesser-known stages of plant autophagy is the degradation of autophagic bodies in vacuoles. Tothis day, no plant vacuolar enzyme has been confirmed to be involved in this process. On the otherhand, several enzymes have been described in yeast (Saccharomyces cerevisiae), including Atg15, thatpossess lipolytic activity. In this preliminary study, which was conducted on isolated embryonicaxes of the white lupin (Lupinus albusL.) and Andean lupin (Lupinus mutabilisSweet), the potentialinvolvement of plant vacuolar lipases in the degradation of autophagic bodies was investigated. Weidentified in transcriptomes (using next-generation sequencing (NGS)) of white and Andean lupinembryonic axes 38 lipases with predicted vacuolar localization, and for three of them, similarities inamino acid sequences with yeast Atg15 were found. A comparative transcriptome analysis of lupinisolated embryonic axes culturedin vitrounder different sucrose and asparagine nutrition, evaluatingthe relations in the levels of the transcripts of lipase genes, was also carried out. A clear decreasein lipase gene transcript levels caused by asparagine, a key amino acid in lupin seed metabolismwhich retards the degradation of autophagic bodies during sugar-starvation-induced autophagy inlupin embryonic axes, was detected. Although the question of whether lipases are involved in thedegradation of autophagic bodies during plant autophagy is still open, our findings strongly supportsuch a hypothesis.
dc.abstract.languageen
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Bioinżynierii
dc.affiliation.instituteKatedra Biochemii i Biotechnologii
dc.contributor.authorWleklik, Karolina
dc.contributor.authorStefaniak, Szymon
dc.contributor.authorNuc, Katarzyna
dc.contributor.authorPietrowska-Borek, Małgorzata
dc.contributor.authorBorek, Sławomir
dc.date.access2025-03-25
dc.date.accessioned2025-03-25T07:45:11Z
dc.date.available2025-03-25T07:45:11Z
dc.date.copyright2023-12-20
dc.date.issued2024
dc.description.abstract<jats:p>Autophagy is a fundamental process for plants that plays a crucial role in maintaining cellular homeostasis and promoting survival in response to various environmental stresses. One of the lesser-known stages of plant autophagy is the degradation of autophagic bodies in vacuoles. To this day, no plant vacuolar enzyme has been confirmed to be involved in this process. On the other hand, several enzymes have been described in yeast (Saccharomyces cerevisiae), including Atg15, that possess lipolytic activity. In this preliminary study, which was conducted on isolated embryonic axes of the white lupin (Lupinus albus L.) and Andean lupin (Lupinus mutabilis Sweet), the potential involvement of plant vacuolar lipases in the degradation of autophagic bodies was investigated. We identified in transcriptomes (using next-generation sequencing (NGS)) of white and Andean lupin embryonic axes 38 lipases with predicted vacuolar localization, and for three of them, similarities in amino acid sequences with yeast Atg15 were found. A comparative transcriptome analysis of lupin isolated embryonic axes cultured in vitro under different sucrose and asparagine nutrition, evaluating the relations in the levels of the transcripts of lipase genes, was also carried out. A clear decrease in lipase gene transcript levels caused by asparagine, a key amino acid in lupin seed metabolism which retards the degradation of autophagic bodies during sugar-starvation-induced autophagy in lupin embryonic axes, was detected. Although the question of whether lipases are involved in the degradation of autophagic bodies during plant autophagy is still open, our findings strongly support such a hypothesis.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,9
dc.description.number1
dc.description.points140
dc.description.versionfinal_published
dc.description.volume25
dc.identifier.doi10.3390/ijms25010090
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2619
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/25/1/90
dc.languageen
dc.pbn.affiliationbiotechnology
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pagesart. 90
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.enasparagine
dc.subject.enAtg15
dc.subject.enautophagy
dc.subject.encarbon starvation
dc.subject.enlupin
dc.subject.enseed embryo
dc.subject.ensucrose
dc.subject.entranscriptomics
dc.subject.envacuole
dc.subject.enyeast
dc.subject.languageen
dc.titleIdentification and Potential Participation of Lipases in Autophagic Body Degradation in Embryonic Axes of Lupin (Lupinus spp.) Germinating Seeds
dc.title.volumeSpecial Issue New Insight into Signaling and Autophagy in Plants 3.0
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume25