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Feline cumulus cells and oocytes show massive accumulation of lipid droplets and upregulation of PLIN2 expression after in vitro maturation

cris.virtual.author-orcid0000-0002-6576-8972
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cris.virtual.author-orcid0000-0003-3489-1684
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
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cris.virtualsource.author-orcidf9afb1ae-f16d-4b00-9b01-bceecb2fb187
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cris.virtualsource.author-orcid4ed4e453-28dc-48e8-864d-49bd28fdc14e
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enThis study investigates lipid content and expression of genes involved in lipid metabolism in feline cumulus cells and oocytes before and after in vitro maturation (IVM). Domestic cats represent valuable models in reproductive research, yet the efficiency of in vitro embryo production remains suboptimal, with contributing factors still under investigation. We characterized lipid droplets (LDs) in oocytes collected from adult queens, both before and after IVM, using confocal microscopy with Bodipy 493/503 staining. We also quantified the expression of four genes involved in regulation of lipid metabolism. Our findings revealed a substantial accumulation of LDs and a significant upregulation of PLIN2 expression in both cumulus cells and oocytes following IVM. The number and total area of LDs and fluorescence intensity increased markedly in oocytes at the MII stage, while the average LD diameter decreased. Similarly, cumulus cells showed an increase in number and total area of LDs post-IVM, suggesting their involvement in oocyte maturation by modulating lipid homeostasis. This study presents the first detailed characterization of lipid droplet dynamics in feline oocytes and cumulus cells, providing insights into metabolic processes during IVM, which are critical for optimizing assisted reproductive technologies in felids.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.contributor.authorSowińska, Natalia
dc.contributor.authorŁechtańska Joanna
dc.contributor.authorGreczka Katarzyna
dc.contributor.authorLechniak Dorota
dc.contributor.authorPawlak, Piotr
dc.date.access2024-12-05
dc.date.accessioned2024-12-05T08:20:49Z
dc.date.available2024-12-05T08:20:49Z
dc.date.copyright2024-11-01
dc.date.issued2025
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,4
dc.description.number15 January 2025
dc.description.points140
dc.description.versionfinal_published
dc.description.volume232
dc.identifier.doi10.1016/j.theriogenealogy.2024.10.031
dc.identifier.issn0093-691X
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2160
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0093691X2400445X?via%3Dihub
dc.languageen
dc.relation.ispartofTheriogenology
dc.relation.pages70-78
dc.rightsClosedAccess
dc.sciencecloudsend
dc.subject.enlipids
dc.subject.encat
dc.subject.enreproduction
dc.subject.engene expression
dc.subject.enconfocal microscopy
dc.subject.enin vitro maturation (IVM)
dc.subject.enassisted reproductive technologies (ART)
dc.titleFeline cumulus cells and oocytes show massive accumulation of lipid droplets and upregulation of PLIN2 expression after in vitro maturation
dc.typeJournalArticle
dspace.entity.typePublication