The metabolic profile of bovine blastocysts is affected by in vitro culture system and the pattern of first zygotic cleavage
| cris.virtual.author-orcid | 0000-0001-8543-0752 | |
| cris.virtual.author-orcid | 0000-0002-7642-0787 | |
| cris.virtual.author-orcid | 0000-0001-6384-791X | |
| cris.virtualsource.author-orcid | 1cbd8c73-bcc9-49b4-a4d0-07e52c3c7001 | |
| cris.virtualsource.author-orcid | fc57cf04-e023-4b05-9241-1c5d72ef234b | |
| cris.virtualsource.author-orcid | 02a0d8c3-755b-4ed1-a428-3e47baa39519 | |
| dc.abstract.en | Time lapse monitoring (TLM) is a commercial system of individual embryo culture based on the well-of-the-well system. It allows for collecting a panel of intrinsic parameters of special importance to non-invasive quality assessment. Besides, a combined analysis of TLM and metabolome data provides a deeper insight into the embryo quality. Two questions have been addressed by this study: (i) whether embryo culture in the Primo Vision affects embryo development and quality compared to the classical system and (ii) whether the first zygotic cleavage (FZC) dysmorphisms affect metabolomic profile of blastocysts. Presumptive zygotes from IVM/IVF oocytes were cultured either in classical drops (pools of 30 embryos, control group) or on Primo Vision plates (16 embryos in separate wells, sharing culture medium, Primo Vision group). The two categories of blastocysts were analysed for relative transcript abundance of five genes (real time PCR; FASN, Hsp70, PLIN2, Bax, Slc2a1) and for metabolite profile (mass spectrometry). Besides, basing on retrospective analysis of the TLM files, the Primo Vision blastocysts were allocated into one of two groups depending on the FZC pattern (normal NC or direct cleavage DC) and analysed for metabolite profiles. The Primo Vision embryos showed higher cleavage rate (p < 0.01) but reduced blastocyst rate (p < 0.05) when compared to the control group. Although the culture system (Primo Vision vs classical) did not affect the relative transcript abundance, the metabolomic analysis revealed different activity of five pathways. Two of them, related to beta-oxidation of fatty acids were up-regulated, whereas the remaining three corresponding to ubiquinone synthesis, metabolism of phenyloalanine and tyrosine as well as vitamin B6 metabolism were down-regulated. The metabolite profiles were however particularly affected by the FZC pattern. The NC and DC blastocysts differed significantly in the activity of 16 metabolic pathways which mainly involved pyruvic acid. The DC embryos displayed a higher level of pyruvic acid (p < 0.05) which may imply disturbances in the switch from lipid to glucose metabolism. Besides, in our opinion, embryos cultured in separate wells in the Primo Vision may face increased ROS level which reduces their developmental potential. Summarizing we underline application of metabolome analysis to embryo quality assessment due to providing a wider insight into embryo response to environmental factors. | |
| dc.affiliation | Wydział Medycyny Weterynaryjnej i Nauk o Zwierzętach | |
| dc.affiliation.institute | Katedra Genetyki i Podstaw Hodowli Zwierząt | |
| dc.contributor.author | Cieślak, Dorota Marta | |
| dc.contributor.author | Sell-Kubiak, Ewa | |
| dc.contributor.author | Warzych-Plejer, Ewelina | |
| dc.date.access | 2025-11-20 | |
| dc.date.accessioned | 2025-11-20T10:59:00Z | |
| dc.date.available | 2025-11-20T10:59:00Z | |
| dc.date.copyright | 2022-05-28 | |
| dc.date.issued | 2022 | |
| dc.description.accesstime | at_publication | |
| dc.description.bibliography | il., bibliogr. | |
| dc.description.finance | publication_nocost | |
| dc.description.financecost | 0,00 | |
| dc.description.if | 2,8 | |
| dc.description.number | August 2022 | |
| dc.description.points | 140 | |
| dc.description.version | final_published | |
| dc.description.volume | 188 | |
| dc.identifier.doi | 10.1016/j.theriogenology.2022.05.021 | |
| dc.identifier.eissn | 1879-3231 | |
| dc.identifier.issn | 0093-691X | |
| dc.identifier.uri | https://sciencerep.up.poznan.pl/handle/item/6019 | |
| dc.identifier.weblink | https://www.sciencedirect.com/science/article/pii/S0093691X22002023 | |
| dc.language | en | |
| dc.relation.ispartof | Theriogenology | |
| dc.relation.pages | 43-51 | |
| dc.rights | CC-BY | |
| dc.sciencecloud | nosend | |
| dc.share.type | OTHER | |
| dc.subject.en | time lapse monitoring | |
| dc.subject.en | first zygotic cleavage | |
| dc.subject.en | metabolomics | |
| dc.subject.en | cattle | |
| dc.title | The metabolic profile of bovine blastocysts is affected by in vitro culture system and the pattern of first zygotic cleavage | |
| dc.type | JournalArticle | |
| dspace.entity.type | Publication | |
| oaire.citation.volume | 188 |