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Effects of pH and palmitic acid on the organization and stability of lutein in the phosphatidylcholine membranes – Spectroscopic studies

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cris.virtual.author-orcid0000-0001-5616-3827
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcida2f42993-2b76-4d53-acc8-61c1b5b10c4e
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enThis paper presents the effect of pH and the presence of palmitic acid (PA) on the organisation and stability of lutein (LUT) in the egg yolk phosphatidylcholine (EYPC) membranes. This is the first known thorough study on the EYPC liposomes formed in different buffers such as acetic at pH 5.0, phosphate at pH 7.4 and borate at pH 9.0. UV–Vis absorption and infra-red (FTIR) spectroscopy were used for molecular studies. Based on analysis of UV–Vis spectra it can be argued that the pH and temperature conditions are crucial for the stability of LUT molecules. After analysing the spectra of samples containing LUT + PA, it can be concluded that the presence of PA in all the applied buffers provides better stability for LUT and perhaps its better solubility. The changes in the absorbance spectrum observed after incubation at 37 °C for 1 h also indicated the beneficial effect of PA presence. Our studies indicate that PA presence in the lipid membranes may have protective effect on LUT, especially at pH 5.0 and that it slows down the degradation process of LUT. Localized pH heterogeneities can induce local dynamical membrane deformations which combined with a certain molecular organization of LUT (such as dimers) can result in lower or higher membrane penetration by LUT molecules.
dc.affiliationWydział Inżynierii Środowiska i Inżynierii Mechanicznej
dc.affiliation.instituteKatedra Inżynierii Biosystemów
dc.contributor.authorReszczyńska, Emilia
dc.contributor.authorSujak, Agnieszka
dc.contributor.authorGruszecki, Wiesław I.
dc.contributor.authorHanaka, Agnieszka
dc.date.access2025-07-04
dc.date.accessioned2025-10-27T10:55:28Z
dc.date.available2025-10-27T10:55:28Z
dc.date.copyright2023-06-03
dc.date.issued2023
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if5,3
dc.description.number15 August 2023
dc.description.points100
dc.description.versionfinal_published
dc.description.volume384
dc.identifier.doi10.1016/j.molliq.2023.122269
dc.identifier.eissn1873-3166
dc.identifier.issn0167-7322
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5470
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0167732223010723
dc.languageen
dc.relation.ispartofJournal of Molecular Liquids
dc.relation.pagesart. 122269
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.enlutein
dc.subject.enmodel membrane
dc.subject.enFTIR-ATR
dc.subject.enUV–Vis spectroscopy
dc.titleEffects of pH and palmitic acid on the organization and stability of lutein in the phosphatidylcholine membranes – Spectroscopic studies
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume384