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A successful defense of the narrow-leafed lupin against anthracnose involves quick and orchestrated reprogramming of oxidation–reduction, photosynthesis and pathogenesis-related genes

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cris.virtual.author-orcid0000-0002-6174-2898
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cris.virtualsource.author-orcid834653b4-c838-4485-840b-59a52aa12b59
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dc.abstract.enNarrow-leafed lupin (NLL, Lupinus angustifolius L.) is a legume plant cultivated for grain production and soil improvement. Worldwide expansion of NLL as a crop attracted various pathogenic fungi, including Colletotrichum lupini causing a devastating disease, anthracnose. Two alleles conferring improved resistance, Lanr1 and AnMan, were exploited in NLL breeding, however, underlying molecular mechanisms remained unknown. In this study, European NLL germplasm was screened with Lanr1 and AnMan markers. Inoculation tests in controlled environment confirmed effectiveness of both resistance donors. Representative resistant and susceptible lines were subjected to differential gene expression profiling. Resistance to anthracnose was associated with overrepresentation of “GO:0006952 defense response”, “GO:0055114 oxidation–reduction process” and “GO:0015979 photosynthesis” gene ontology terms. Moreover, the Lanr1 (83A:476) line revealed massive transcriptomic reprogramming quickly after inoculation, whereas other lines showed such a response delayed by about 42 h. Defense response was associated with upregulation of TIR-NBS, CC-NBS-LRR and NBS-LRR genes, pathogenesis-related 10 proteins, lipid transfer proteins, glucan endo-1,3-beta-glucosidases, glycine-rich cell wall proteins and genes from reactive oxygen species pathway. Early response of 83A:476, including orchestrated downregulation of photosynthesis-related genes, coincided with the successful defense during fungus biotrophic growth phase, indicating effector-triggered immunity. Mandelup response was delayed and resembled general horizontal resistance.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Bioinżynierii
dc.affiliation.instituteKatedra Genetyki i Hodowli Roślin
dc.contributor.authorKsiążkiewicz, Michał
dc.contributor.authorRychel-Bielska, Sandra
dc.contributor.authorPlewiński, Piotr
dc.contributor.authorBielski, Wojciech
dc.contributor.authorNuc, Maria
dc.contributor.authorKozak, Bartosz
dc.contributor.authorKrajewski, Paweł
dc.contributor.authorJędryczka, Małgorzata
dc.date.access2026-01-28
dc.date.accessioned2026-02-09T08:55:16Z
dc.date.available2026-02-09T08:55:16Z
dc.date.copyright2022-05-17
dc.date.issued2022
dc.description.abstract<jats:title>Abstract</jats:title><jats:p>Narrow-leafed lupin (NLL, <jats:italic>Lupinus angustifolius</jats:italic> L.) is a legume plant cultivated for grain production and soil improvement. Worldwide expansion of NLL as a crop attracted various pathogenic fungi, including <jats:italic>Colletotrichum lupini</jats:italic> causing a devastating disease, anthracnose. Two alleles conferring improved resistance, <jats:italic>Lanr1</jats:italic> and <jats:italic>AnMan</jats:italic>, were exploited in NLL breeding, however, underlying molecular mechanisms remained unknown. In this study, European NLL germplasm was screened with <jats:italic>Lanr1</jats:italic> and <jats:italic>AnMan</jats:italic> markers. Inoculation tests in controlled environment confirmed effectiveness of both resistance donors. Representative resistant and susceptible lines were subjected to differential gene expression profiling. Resistance to anthracnose was associated with overrepresentation of “GO:0006952 defense response”, “GO:0055114 oxidation–reduction process” and “GO:0015979 photosynthesis” gene ontology terms. Moreover, the <jats:italic>Lanr1</jats:italic> (83A:476) line revealed massive transcriptomic reprogramming quickly after inoculation, whereas other lines showed such a response delayed by about 42 h. Defense response was associated with upregulation of TIR-NBS, CC-NBS-LRR and NBS-LRR genes, pathogenesis-related 10 proteins, lipid transfer proteins, glucan endo-1,3-beta-glucosidases, glycine-rich cell wall proteins and genes from reactive oxygen species pathway. Early response of 83A:476, including orchestrated downregulation of photosynthesis-related genes, coincided with the successful defense during fungus biotrophic growth phase, indicating effector-triggered immunity. Mandelup response was delayed and resembled general horizontal resistance.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,6
dc.description.points140
dc.description.versionfinal_published
dc.description.volume12
dc.identifier.doi10.1038/s41598-022-12257-7
dc.identifier.issn2045-2322
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/7254
dc.identifier.weblinkhttps://www.nature.com/articles/s41598-022-12257-7
dc.languageen
dc.relation.ispartofScientific Reports
dc.relation.pagesart. 8164
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.titleA successful defense of the narrow-leafed lupin against anthracnose involves quick and orchestrated reprogramming of oxidation–reduction, photosynthesis and pathogenesis-related genes
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume12