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Nitric Oxide Implication in Potato Immunity to Phytophthora infestans via Modifications of Histone H3/H4 Methylation Patterns on Defense Genes

cris.virtual.author-orcid0000-0002-1624-0514
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cris.virtual.author-orcid0000-0003-2281-223X
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cris.virtualsource.author-orcid08471968-0a68-4f5f-a975-03aa3a75c3ac
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cris.virtualsource.author-orcid4e2dc392-8cf9-4b89-a596-333bcc45d7cd
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dc.abstract.enNitric oxide (NO) is an essential redox-signaling molecule operating in many physiological and pathophysiological processes. However, evidence on putative NO engagement in plant immunity by affecting defense gene expressions, including histone modifications, is poorly recognized. Exploring the effect of biphasic NO generation regulated by S-nitrosoglutathione reductase (GNSOR) activity after avr Phytophthora infestans inoculation, we showed that the phase of NO decline at 6 h post-inoculation (hpi) was correlated with the rise of defense gene expressions enriched in the TrxG-mediated H3K4me3 active mark in their promoter regions. Here, we report that arginine methyltransferase PRMT5 catalyzing histone H4R3 symmetric dimethylation (H4R3sme2) is necessary to ensure potato resistance to avr P. infestans. Both the pathogen and S-nitrosoglutathione (GSNO) altered the methylation status of H4R3sme2 by transient reduction in the repressive mark in the promoter of defense genes, R3a and HSR203J (a resistance marker), thereby elevating their transcription. In turn, the PRMT5-selective inhibitor repressed R3a expression and attenuated the hypersensitive response to the pathogen. In conclusion, we postulate that lowering the NO level (at 6 hpi) might be decisive for facilitating the pathogen-induced upregulation of stress genes via histone lysine methylation and PRMT5 controlling potato immunity to late blight.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Bioinżynierii
dc.affiliation.instituteKatedra Fizjologii Roślin
dc.contributor.authorDrozda, Andżelika
dc.contributor.authorKurpisz, Barbara
dc.contributor.authorArasimowicz-Jelonek, Magdalena
dc.contributor.authorKuźnicki, Daniel
dc.contributor.authorJagodzik, Przemysław
dc.contributor.authorGuan, Yufeng
dc.contributor.authorFloryszak-Wieczorek, Jolanta
dc.date.access2025-08-27
dc.date.accessioned2025-08-27T10:33:36Z
dc.date.available2025-08-27T10:33:36Z
dc.date.copyright2022-04-06
dc.date.issued2022
dc.description.abstract<jats:p>Nitric oxide (NO) is an essential redox-signaling molecule operating in many physiological and pathophysiological processes. However, evidence on putative NO engagement in plant immunity by affecting defense gene expressions, including histone modifications, is poorly recognized. Exploring the effect of biphasic NO generation regulated by S-nitrosoglutathione reductase (GNSOR) activity after avr Phytophthora infestans inoculation, we showed that the phase of NO decline at 6 h post-inoculation (hpi) was correlated with the rise of defense gene expressions enriched in the TrxG-mediated H3K4me3 active mark in their promoter regions. Here, we report that arginine methyltransferase PRMT5 catalyzing histone H4R3 symmetric dimethylation (H4R3sme2) is necessary to ensure potato resistance to avr P. infestans. Both the pathogen and S-nitrosoglutathione (GSNO) altered the methylation status of H4R3sme2 by transient reduction in the repressive mark in the promoter of defense genes, R3a and HSR203J (a resistance marker), thereby elevating their transcription. In turn, the PRMT5-selective inhibitor repressed R3a expression and attenuated the hypersensitive response to the pathogen. In conclusion, we postulate that lowering the NO level (at 6 hpi) might be decisive for facilitating the pathogen-induced upregulation of stress genes via histone lysine methylation and PRMT5 controlling potato immunity to late blight.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_research
dc.description.financecost12000,00
dc.description.if5,6
dc.description.number7
dc.description.points140
dc.description.versionfinal_published
dc.description.volume23
dc.identifier.doi10.3390/ijms23074051
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4430
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/23/7/4051
dc.languageen
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pagesart. 4051
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.ennitric oxide
dc.subject.enstress-responsive gene regulation
dc.subject.enhistone lysine
dc.subject.enarginine methylation
dc.subject.enpotato hypersensitive response
dc.subject.enlate blight
dc.titleNitric Oxide Implication in Potato Immunity to Phytophthora infestans via Modifications of Histone H3/H4 Methylation Patterns on Defense Genes
dc.title.volumeSpecial Issue Advanced Research in Plant Responses to Environmental Stresses 2.0
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue7
oaire.citation.volume23
project.funder.name2017/25/B/NZ9/00905