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MOTS-c Impact on Muscle Cell Differentiation and Metabolism Across Fiber Types

cris.lastimport.scopus2025-10-23T06:56:54Z
cris.virtual.author-orcid0000-0002-0081-7585
cris.virtual.author-orcid0000-0002-7182-6905
cris.virtual.author-orcid0000-0003-0715-0223
cris.virtual.author-orcid0000-0002-0412-5578
cris.virtual.author-orcid0000-0001-5209-2232
cris.virtual.author-orcid0000-0001-9792-1145
cris.virtualsource.author-orcidfa9a05b6-07fa-47e8-ae3d-8929da6881f3
cris.virtualsource.author-orcid325904ea-6775-4d8a-b846-0104d9ec20ca
cris.virtualsource.author-orcid0c22ceb5-e5c6-4f5d-a0fd-8ec91a16efa9
cris.virtualsource.author-orcid1d7e7a9f-dfd0-4ed2-9512-a9aa9cf4ba27
cris.virtualsource.author-orcid59d1337d-8f96-43d3-bd28-674f4db5b81d
cris.virtualsource.author-orcid1815dbc7-82a3-4ce5-8dc9-bbe17826792b
dc.abstract.enMOTS-c belongs to a group of mitochondrial peptides involved in metabolic processes in the body. This peptide has garnered increasing attention since its discovery in 2015 because of its potential to ameliorate metabolic parameters in animals with diabetes or insulin resistance. MOTS-c is involved in muscle metabolism; however, little is known about its role in fiber differentiation. Materials: We conducted a study to explore the effect of MOTS-c on cellular processes using the C2C12 and L6 cell lines, representing different metabolic types of muscle fibers. The research methods were real-time PCR, Western blot, and lipid accumulation measurement. Results: Notably, our investigations revealed that MOTS-c increased the survival of C2C12 cells at doses of 10 and 100 nM (p<0.01) and stimulated the phosphorylation of extracellular signal-regulated kinase within 5 min of incubation (p<0.05). Remarkably, these effects were not observed in L6 cells; however, both cell lines showed a reduced rate of proliferation. Furthermore, MOTS-c promotes the differentiation of C2C12 cells by increasing the expression of muscle regulatory factors, but it does not produce such an effect in L6 cells. Additionally, cells were treated with physiological concentrations of free fatty acids and MOTS-c, unveiling an augmentation in lipid accumulation observed in L6 cells and a decrease in lipid accumulation in C2C12 cells. Conclusion: In conclusion, our findings have suggested a diverse response to MOTS-c depending on the type of muscle fibers, particularly in the domains of survival, cell differentiation, and lipid accumulation.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.contributor.authorLeciejewska, Natalia
dc.contributor.authorPruszyńska-Oszmałek, Ewa
dc.contributor.authorKołodziejski, Paweł Antoni
dc.contributor.authorSzczepankiewicz, Dawid
dc.contributor.authorNogowski, Leszek
dc.contributor.authorSassek, Maciej
dc.date.access2025-05-09
dc.date.accessioned2025-05-08T10:47:51Z
dc.date.available2025-05-08T10:47:51Z
dc.date.copyright2025-01-21
dc.date.issued2025
dc.description.abstract<jats:p>Background/Aims: MOTS-c belongs to a group of mitochondrial peptides involved in metabolic processes in the body. This peptide has garnered increasing attention since its discovery in 2015 because of its potential to ameliorate metabolic parameters in animals with diabetes or insulin resistance. MOTS-c is involved in muscle metabolism; however, little is known about its role in fiber differentiation. Materials: We conducted a study to explore the effect of MOTS-c on cellular processes using the C2C12 and L6 cell lines, representing different metabolic types of muscle fibers. The research methods were real-time PCR, Western blot, and lipid accumulation measurement. Results: &gt;Notably, our investigations revealed that MOTS-c increased the survival of C2C12 cells at doses of 10 and 100 nM (p&lt;0.01) and stimulated the phosphorylation of extracellular signal-regulated kinase within 5 min of incubation (p&lt;0.05). Remarkably, these effects were not observed in L6 cells; however, both cell lines showed a reduced rate of proliferation. Furthermore, MOTS-c promotes the differentiation of C2C12 cells by increasing the expression of muscle regulatory factors, but it does not produce such an effect in L6 cells. Additionally, cells were treated with physiological concentrations of free fatty acids and MOTS-c, unveiling an augmentation in lipid accumulation observed in L6 cells and a decrease in lipid accumulation in C2C12 cells. Conclusion: In conclusion, our findings have suggested a diverse response to MOTS-c depending on the type of muscle fibers, particularly in the domains of survival, cell differentiation, and lipid accumulation.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_research
dc.description.financecost7880,92
dc.description.if2,5
dc.description.number1
dc.description.points140
dc.description.versionfinal_published
dc.description.volume59
dc.identifier.doi10.33594/000000755
dc.identifier.eissn1421-9778
dc.identifier.issn1015-8987
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2762
dc.identifier.weblinkhttps://cellphysiolbiochem.com/Articles/000755/
dc.languageen
dc.relation.ispartofCellular Physiology and Biochemistry
dc.relation.pages34-46
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.enMOTS-c
dc.subject.enmuscle cells
dc.subject.endifferentiation
dc.subject.enmuscle regulatory factors
dc.titleMOTS-c Impact on Muscle Cell Differentiation and Metabolism Across Fiber Types
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume59