Isoflavones and probiotics effect on bone calcium and bone cells in rats

cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-6734-6271
cris.virtual.author-orcid0000-0001-8417-2245
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cris.virtual.author-orcid0000-0002-7182-6905
cris.virtual.author-orcid0000-0001-9792-1145
cris.virtual.author-orcid0000-0002-0937-8427
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cris.virtualsource.author-orcid9510fdb3-2b25-4ccb-baf7-442036593f18
cris.virtualsource.author-orcidedf63522-bb89-4788-a8d3-9c3f3858875b
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cris.virtualsource.author-orcid325904ea-6775-4d8a-b846-0104d9ec20ca
cris.virtualsource.author-orcid1815dbc7-82a3-4ce5-8dc9-bbe17826792b
cris.virtualsource.author-orcid31a85399-18e5-444d-b0c6-96b441dd2551
dc.abstract.enIsoflavones and probiotics have shown the therapeutic potential to alter calcium absorption and bone cell metabolism. This study sought to ascertain the effect of isoflavones and probiotics on calcium status and bone health in healthy female rats. Forty-eight adult female Wistar rats were grouped and fed: a standard diet (control); and standard diets with tempeh; soy; daidzein and genistein; Lactobacillus acidophilus; and a combination of daidzein, genistein, and L. acidophilus. The biochemical serum parameters, such as alanine transaminase, aspartate transaminase, glucose, and triacylglycerol concentrations, were measured, and calcium contents in tissues were determined. After staining the bone with hematoxylin and eosin, the number of osteoblasts, osteocytes, and the percentage of bone marrow adipocytes were counted. Compared with the control group, the soy group showed a significantly lower triacylglycerol concentration. The L. acidophilus group considerably increased the calcium content in the femoral bone. The daidzein and genistein, L. acidophilus, and a combination of daidzein, genistein, and L. acidophilus groups showed significantly lower calcium contents in the heart and kidneys. The daidzein and genistein group significantly enhanced the number of osteoblasts and osteocytes. A substantial inverse correlation was observed between calcium contents in kidneys and osteoblasts. In conclusion, the combination of daidzein, genistein, and L. acidophilus may improve bone calcium concentrations and bone cells. However, no synergistic effect between isoflavones and probiotics was detected in this study.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Żywienia Człowieka i Dietetyki
dc.affiliation.instituteKatedra Technologii Żywności Pochodzenia Roślinnego
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.contributor.authorHarahap, Iskandar Azmy
dc.contributor.authorKuligowski, Maciej
dc.contributor.authorSchmidt, Marcin
dc.contributor.authorKurzawa, Paweł
dc.contributor.authorPruszyńska-Oszmałek, Ewa
dc.contributor.authorSassek, Maciej
dc.contributor.authorSuliburska, Joanna
dc.date.access2025-06-25
dc.date.accessioned2025-10-07T12:37:04Z
dc.date.available2025-10-07T12:37:04Z
dc.date.copyright2023-05-29
dc.date.issued2023
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3,4
dc.description.number6 (June 2023)
dc.description.points40
dc.description.versionfinal_published
dc.description.volume9
dc.identifier.doi10.1016/j.heliyon.2023.e16801
dc.identifier.eissn2405-8440
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5260
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S2405844023040082
dc.languageen
dc.relation.ispartofHeliyon
dc.relation.pagesart. e16801
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enisoflavones
dc.subject.enLactobacillus acidophilus
dc.subject.encalcium status
dc.subject.enbone cells
dc.subject.enrats
dc.titleIsoflavones and probiotics effect on bone calcium and bone cells in rats
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue6
oaire.citation.volume9