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Biomarkers of oxidative stress, biochemical changes, and the activity of lysosomal enzymes in the livers of rainbow trout (Oncorhynchus mykiss Walbaum) vaccinated against yersiniosis before a Yersinia ruckeri challenge

cris.lastimport.scopus2025-10-23T06:57:08Z
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dc.abstract.enIntroduction: This study aimed to evaluate biomarkers of oxidative stress (2-thiobarbituric acid reactive substances, aldehyde and ketone derivatives of oxidatively modified proteins and total antioxidant capacity), the activity of antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase), that of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase and acid phosphatase) and changes in biochemical parameters (alanine aminotransferase, aspartate aminotransferase, de Ritis ratio, lactate dehydrogenase activity, lactate and pyruvate levels and their ratio) in the liver tissue of fish that were vaccinated against enteric redmouth disease and challenged with its causative agent, the bacterium Yersinia ruckeri. Material and Methods: The vaccine was administered orally to trout, some of which were challenged with Y. ruckeri 61 days later. For comparison, unvaccinated and unchallenged trout and unvaccinated and challenged trout were also evaluated. Results: In the unvaccinated fish, infection with Y. ruckeri disrupted the pro-oxidant/antioxidant balance, led to a significant increase in lipid peroxidation and oxidative modification of proteins, decreased total antioxidant capacity and significantly increased the activity of lysosomal enzymes. In vaccinated fish, the Y. ruckeri challenge increased the activity of glutathione-related enzymes and decreased lipid peroxidation, anaerobic metabolism and the activity of lysosomal enzymes in fish livers relative to the unvaccinated and challenged group. In contrast, these parameters increased after the Y. ruckeri challenge in unvaccinated trout relative to those in the untreated group. Conclusion: Vaccination exerted a protective effect during the Y. ruckeri challenge and had no adverse effect on fish livers.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Nauk Przedklinicznych i Chorób Zakaźnych
dc.contributor.authorKurhaluk, Natalia
dc.contributor.authorGrudniewska, Joanna
dc.contributor.authorPękala-Safińska, Agnieszka
dc.contributor.authorPajdak-Czaus, Joanna
dc.contributor.authorTerech-Majewska, Elżbieta
dc.contributor.authorPlatt-Samoraj, Aleksandra
dc.contributor.authorTkaczenko, Halina
dc.date.access2024-10-16
dc.date.accessioned2024-10-29T10:10:24Z
dc.date.available2024-10-29T10:10:24Z
dc.date.copyright2024-09-23
dc.date.issued2024
dc.description.abstract<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Introduction</jats:title> <jats:p>This study aimed to evaluate biomarkers of oxidative stress (2-thiobarbituric acid reactive substances, aldehyde and ketone derivatives of oxidatively modified proteins and total antioxidant capacity), the activity of antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase), that of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase and acid phosphatase) and changes in biochemical parameters (alanine aminotransferase, aspartate aminotransferase, de Ritis ratio, lactate dehydrogenase activity, lactate and pyruvate levels and their ratio) in the liver tissue of fish that were vaccinated against enteric redmouth disease and challenged with its causative agent, the bacterium <jats:italic>Yersinia ruckeri</jats:italic>.</jats:p> </jats:sec> <jats:sec> <jats:title>Material and Methods</jats:title> <jats:p>The vaccine was administered orally to trout, some of which were challenged with <jats:italic>Y. ruckeri</jats:italic> 61 days later. For comparison, unvaccinated and unchallenged trout and unvaccinated and challenged trout were also evaluated.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>In the unvaccinated fish, infection with <jats:italic>Y. ruckeri</jats:italic> disrupted the pro-oxidant/antioxidant balance, led to a significant increase in lipid peroxidation and oxidative modification of proteins, decreased total antioxidant capacity and significantly increased the activity of lysosomal enzymes. In vaccinated fish, the <jats:italic>Y. ruckeri</jats:italic> challenge increased the activity of glutathione-related enzymes and decreased lipid peroxidation, anaerobic metabolism and the activity of lysosomal enzymes in fish livers relative to the unvaccinated and challenged group. In contrast, these parameters increased after the <jats:italic>Y. ruckeri</jats:italic> challenge in unvaccinated trout relative to those in the untreated group.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>Vaccination exerted a protective effect during the <jats:italic>Y. ruckeri</jats:italic> challenge and had no adverse effect on fish livers.</jats:p> </jats:sec>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0.00
dc.description.if1,3
dc.description.number3
dc.description.points140
dc.description.versionfinal_published
dc.description.volume68
dc.identifier.doi10.2478/jvetres-2024-0050
dc.identifier.eissn2450-8608
dc.identifier.issn2450-7393
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/1951
dc.identifier.weblinkhttps://sciendo.com/pl/article/10.2478/jvetres-2024-0050?tab=aurhors
dc.languageen
dc.relation.ispartofJournal of Veterinary Research (Poland)
dc.relation.pages325-336
dc.rightsCC-BY-NC-ND
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.enYersinia ruckeri
dc.subject.enchallenge model
dc.subject.enrainbow trout
dc.subject.enoxidative stress
dc.subject.enantioxidant defences
dc.subject.enlysosomal enzymes
dc.titleBiomarkers of oxidative stress, biochemical changes, and the activity of lysosomal enzymes in the livers of rainbow trout (Oncorhynchus mykiss Walbaum) vaccinated against yersiniosis before a Yersinia ruckeri challenge
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue3
oaire.citation.volume68