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Caffeine enhances lipolysis in primary rat adipocytes via adenosine A1 receptor pathway

cris.lastimport.scopus2025-10-23T06:57:09Z
cris.virtual.author-orcid0000-0002-6828-0534
cris.virtual.author-orcid0000-0002-3651-0972
cris.virtual.author-orcid0000-0003-4059-1362
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cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-9067-6682
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cris.virtualsource.author-orciddaf06652-dcef-4aa8-b66a-bdb14028e85d
cris.virtualsource.author-orcid652e209d-434a-47b3-b5fb-64c0aac5a84c
cris.virtualsource.author-orcid9118c61a-b066-4efc-a7af-486f9ebf9021
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cris.virtualsource.author-orciddb8a4db7-12b5-4442-8968-d898d1ac259e
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cris.virtualsource.author-orcidff566057-8099-4bac-bd4c-896c46beaa7c
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enCaffeine is a well-known compound broadly consumed by humans as an ingredient in beverages. Its ingestion is associated with numerous beneficial effects on the organism. Caffeine may, among others, reduce adipose tissue accumulation. However, the mechanism underlying this action is poorly elucidated. Lipolysis is a relevant process directly related to adipocyte metabolism. The present study explored the short-term (2-h) effects of 1 mM caffeine on the lipolytic activity of isolated adipocytes. Lipolysis was measured as the quantity of glycerol released from cells. The expression of lipolysis-related genes was also determined at the mRNA level. It was shown that caffeine significantly increased lipolysis induced by epinephrine (an adrenergic receptor agonist), forskolin (a direct activator of adenylate cyclase (AC)), and dibutyryl-cAMP (a direct stimulator of protein kinase A (PKA)). However, the lipolytic response of fat cells to DPCPX (an adenosine A1 receptor antagonist) was unchanged by caffeine. Moreover, caffeine failed to affect lipolysis stimulated by the combination of DPCPX with epinephrine, DPCPX with DB-cAMP and DPCPX with forskolin. Additionally, the stimulatory effect of caffeine on epinephrine-induced glycerol release was suppressed by H-89 (a direct inhibitor of PKA). Caffeine slightly increased the cAMP content (a signaling molecule increasing the lipolytic process) in adipocytes. However, the expression of analyzed genes was not significantly affected in the presence of the tested compound. The results show that caffeine promotes lipolysis in primary rat adipocytes by inhibiting the adenosine A1 receptor signaling pathway. The pharmacological suppression of PKA abolishes the stimulatory effect. Increased lipolysis in the presence of caffeine contributes to reduced lipid accumulation in adipocytes
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.contributor.authorKonieczna, Klaudia
dc.contributor.authorSzkudelska, Katarzyna
dc.contributor.authorWojciechowicz, Tatiana
dc.contributor.authorFiedorowicz, Joanna
dc.contributor.authorKrążek, Małgorzata
dc.contributor.authorSkrzypski, Marek
dc.contributor.authorFakruzzaman, Md.
dc.contributor.authorSzkudelski, Tomasz
dc.date.access2025
dc.date.accessioned2025-09-18T12:12:22Z
dc.date.available2025-09-18T12:12:22Z
dc.date.copyright2025-08-31
dc.date.issued2025
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financeother
dc.description.financecost2500,00
dc.description.if1,7
dc.description.number4
dc.description.points100
dc.description.versionfinal_published
dc.description.volume76
dc.identifier.doi10.26402/jpp.2025.4.10
dc.identifier.eissn1899-1505
dc.identifier.issn0867-5910
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4925
dc.identifier.weblinkhttps://www.jpp.krakow.pl
dc.languageen
dc.relation.ispartofJournal of Physiology and Pharmacology
dc.relation.pages473-483
dc.rightsOther
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.encaffeine
dc.subject.enadipocytes
dc.subject.enlipolysis
dc.subject.englycerol
dc.subject.enreceptor
dc.subject.enadenosine A1
dc.subject.enprotein kinase A
dc.subject.enphosphodiesterases
dc.subject.enepinephrine
dc.titleCaffeine enhances lipolysis in primary rat adipocytes via adenosine A1 receptor pathway
dc.typeJournalArticle
dspace.entity.typePublication