Simple item page
 

Maternal cafeteria diet influences kisspeptin (Kiss1), kisspeptin receptor(Gpr54), and sirtuin (Sirt1) genes, hormonal and metabolic profiles, and reproductive functions in rat offspring in a sex-specific manner

cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-1041-3576
cris.virtual.author-orcid0000-0003-4765-4821
cris.virtual.author-orcid0000-0002-8798-991X
cris.virtual.author-orcid0000-0002-7182-6905
cris.virtual.author-orcid0000-0002-1370-7625
cris.virtual.author-orcid0000-0002-2045-0709
cris.virtual.author-orcid0000-0001-6981-9653
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcidb1f9951f-b018-4795-8cff-c7188dd69448
cris.virtualsource.author-orcidb8e6f1ba-873f-4d37-b5e8-2e71c3504f2c
cris.virtualsource.author-orcid7d619370-fc16-4ebc-8bfa-6738a36749ee
cris.virtualsource.author-orcid325904ea-6775-4d8a-b846-0104d9ec20ca
cris.virtualsource.author-orcid86de7053-c0cc-4a23-960c-fd3908659acc
cris.virtualsource.author-orcid20ac3fe1-f398-4ef7-ab0b-a6a20460f69f
cris.virtualsource.author-orcid06d96226-5dc5-4667-a85b-254ac606fda1
dc.abstract.enKisspeptin (KP, encoded by Kiss1, binding to the Gpr54 receptor) is a neuropeptide conveying information on the metabolic status to the hypothalamic–pituitary–gonadal axis. KP acts together with dynorphin A (encoded by Pdyn) and neurokinin B (encoded by Tac2) to regulate reproduction. KP is crucial for the onset of puberty and is under the control of sirtuin (encoded by Sirt1). We hypothesize that the maternal cafeteria (CAF) diet has adverse effects on the offspring’s hormonal, metabolic, and reproductive functions due to sex-specific alterations in the expression of Kiss1, Gpr54, Pdyn, Tac2, and Sirt1 in the hypothalamus, and Kiss1, Gpr54, and Sirt1 in the liver. Rats were fed a CAF diet before pregnancy, during pregnancy, and during lactation. The vaginal opening was monitored. Offspring were sacrificed in three age points: PND 30, PND 35, and PND 60 (females) and PND 40, PND 45, and PND 60 (males). Their metabolic and hormonal status was assessed. mRNA for Kiss1, Gpr54, Pdyn, Tac2, and Sirt1 were measured by real-time PCR in the hypothalamus and/or livers. We found that CAF offspring had lower weight and altered body composition; increased cholesterol and triglyceride levels, sex-specific changes in glucose and insulin levels; sex-dependent changes in Sirt1/Kiss1 mRNA ratio in the hypothalamus; sex-specific alterations in Kiss1 and Sirt1 mRNA in the liver with more diversity in males; and a delayed puberty onset in females. We concluded that the mother’s CAF diet leads to sex-specific alterations in metabolic and reproductive outcomes via Kiss1/Gpr54 and Sirt1 systems in offspring.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.affiliation.instituteKatedra Żywienia Człowieka i Dietetyki
dc.affiliation.instituteKatedra Zoologii
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.contributor.authorMatuszewska, Julia
dc.contributor.authorNowacka-Woszuk, Joanna
dc.contributor.authorRadziejewska, Anna Maria
dc.contributor.authorGrzęda, Emilia
dc.contributor.authorPruszyńska-Oszmałek, Ewa
dc.contributor.authorDylewski, Łukasz
dc.contributor.authorChmurzyńska, Agata
dc.contributor.authorŚliwowska, Joanna Helena
dc.date.access2025-06-06
dc.date.accessioned2025-09-15T06:23:36Z
dc.date.available2025-09-15T06:23:36Z
dc.date.copyright2023-09-04
dc.date.issued2023
dc.description.abstract<jats:title>Abstract</jats:title> <jats:p>Kisspeptin (KP, encoded by Kiss1, binding to the Gpr54 receptor) is a neuropeptide conveying information on the metabolic status to the hypothalamic–pituitary–gonadal axis. KP acts together with dynorphin A (encoded by Pdyn) and neurokinin B (encoded by Tac2) to regulate reproduction. KP is crucial for the onset of puberty and is under the control of sirtuin (encoded by Sirt1). We hypothesize that the maternal cafeteria (CAF) diet has adverse effects on the offspring’s hormonal, metabolic, and reproductive functions due to sex-specific alterations in the expression of Kiss1, Gpr54, Pdyn, Tac2, and Sirt1 in the hypothalamus, and Kiss1, Gpr54, and Sirt1 in the liver. Rats were fed a CAF diet before pregnancy, during pregnancy, and during lactation. The vaginal opening was monitored. Offspring were sacrificed in three age points: PND 30, PND 35, and PND 60 (females) and PND 40, PND 45, and PND 60 (males). Their metabolic and hormonal status was assessed. mRNA for Kiss1, Gpr54, Pdyn, Tac2, and Sirt1 were measured by real-time PCR in the hypothalamus and/or livers. We found that CAF offspring had lower weight and altered body composition; increased cholesterol and triglyceride levels, sex-specific changes in glucose and insulin levels; sex-dependent changes in Sirt1/Kiss1 mRNA ratio in the hypothalamus; sex-specific alterations in Kiss1 and Sirt1 mRNA in the liver with more diversity in males; and a delayed puberty onset in females. We concluded that the mother’s CAF diet leads to sex-specific alterations in metabolic and reproductive outcomes via Kiss1/Gpr54 and Sirt1 systems in offspring.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3,1
dc.description.number5 (November 2023)
dc.description.points200
dc.description.versionfinal_published
dc.description.volume109
dc.identifier.doi10.1093/biolre/ioad101
dc.identifier.eissn1529-7268
dc.identifier.issn0006-3363
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4765
dc.identifier.weblinkhttp://academic.oup.com/biolreprod/article/109/5/654/7259346
dc.languageen
dc.relation.ispartofBiology of Reproduction
dc.relation.pages654-668
dc.rightsCC-BY-NC
dc.sciencecloudsend
dc.share.typeOTHER
dc.subject.enobesity
dc.subject.enpuberty
dc.subject.encafeteriadiet
dc.subject.enkisspeptin
dc.subject.enKNDyneurons
dc.subject.enliver
dc.subject.enhypothalamus
dc.subject.enSIRT1
dc.titleMaternal cafeteria diet influences kisspeptin (Kiss1), kisspeptin receptor(Gpr54), and sirtuin (Sirt1) genes, hormonal and metabolic profiles, and reproductive functions in rat offspring in a sex-specific manner
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue5
oaire.citation.volume109