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Effect of a high-fat high-fructose diet on the composition of the intestinal microbiota and its association with metabolic and anthropometric parameters in a letrozole-induced mouse model of polycystic ovary syndrome

cris.virtual.author-orcid0000-0002-7402-1328
cris.virtual.author-orcid0000-0002-7182-6905
cris.virtual.author-orcid0000-0003-0715-0223
cris.virtual.author-orcid0000-0001-7367-9120
cris.virtual.author-orcid0000-0002-2268-9326
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid59851afa-3d5d-435f-95ee-4130cbd454a0
cris.virtualsource.author-orcid325904ea-6775-4d8a-b846-0104d9ec20ca
cris.virtualsource.author-orcid0c22ceb5-e5c6-4f5d-a0fd-8ec91a16efa9
cris.virtualsource.author-orcid964f07c7-daee-4dea-80f5-0d05dc86444e
cris.virtualsource.author-orcid190a1224-971b-4b6e-a791-aa08ba93ca89
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enObjective:It has been suggested that dysbiosis of the gut microbiota is associated with the pathogenesis of Polycystic Ovary Syndrome (PCOS), and that improper diet can aggravate these changes. This study thus aimed to investigate the effects of a high-fat/high-fructose (HF/HFr) diet on the gut microbial community and their metabolites in prepubertal female mice with letrozole (LET)-induced PCOS. We also tested the correlations between the relative abundance of microbial taxa and selected PCOS parameters. Research methods & procedures: Thirty-two C57BL/6 mice were randomly divided into four groups (n = 8) and implanted with LET or a placebo, with simultaneous administration of a HF/HFr diet or standard diet (StD) for 5 wk. The blood and intestinal contents were collected after the sacrifice. Results: Placebo + HF/HFr and LET + HF/HFr had significantly higher microbial alpha diversity than either group fed StD. The LET-implanted mice fed StD had a significantly higher abundance of Prevotellaceae_UCG-001 than the placebo mice fed StD. Both groups fed the HF/HFr diet had significantly lower fecal levels of short-chain fatty acids than the placebo mice fed StD, while the LET + HF/HFr animals had significantly higher concentrations of lipopolysaccharides in blood serum than either the placebo or LET mice fed StD. Opposite correlations were observed between Turicibacter and Lactobacillus and the lipid profile, Conclusion: HF/HFr diet had a much stronger effect on the composition of the intestinal microbiota of prepubertal mice than LET itself.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Żywienia Człowieka i Dietetyki
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.article.number112450
dc.contributor.authorPieczyńska-Zając, Joanna Maria
dc.contributor.authorMalinowska, Anna Maria
dc.contributor.authorPruszyńska-Oszmałek, Ewa
dc.contributor.authorKołodziejski, Paweł Antoni
dc.contributor.authorDrzymała-Czyż, Sławomira
dc.contributor.authorBajerska, Joanna
dc.date.access2024-07-17
dc.date.accessioned2024-07-17T09:57:00Z
dc.date.available2024-07-17T09:57:00Z
dc.date.copyright2024-04-25
dc.date.issued2024
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3.2
dc.description.numberAugust 2024
dc.description.points140
dc.description.reviewreview
dc.description.versionfinal_published
dc.description.volume124
dc.identifier.doi10.1016/j.nut.2024.112450
dc.identifier.eissn1873-1244
dc.identifier.issn0899-9007
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/1595
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S089990072400100X
dc.languageen
dc.relation.ispartofNutrition
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.enintestinal microbiota
dc.subject.enshort-chain fatty acids
dc.subject.enlipopolysaccharide
dc.subject.enreproductive disorders
dc.titleEffect of a high-fat high-fructose diet on the composition of the intestinal microbiota and its association with metabolic and anthropometric parameters in a letrozole-induced mouse model of polycystic ovary syndrome
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume124
project.funder.nameinne