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Post-Column Guanosine Addition as a Screening Tool in the Search for Effective G-Quadruplex Binders - A Case Study of Achyrocline satureioides Phenolic Compounds

cris.lastimport.scopus2025-10-23T06:54:17Z
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dc.abstract.enPolyphenols make a numerous and diverse group of plant secondary metabolites exhibiting remarkable anticancer activities, often attributed to their G-quadruplex binding properties. Therefore, there is a need to develop a high–throughput screening assay which would permit the evaluation of polyphenols’ binding properties toward G-quadruplex. As deoxyguanosine and guanosine are essential and key building blocks of G-quadruplexes, the stabilities of their adducts with polyphenols may reflect the stabilities of polyphenols–G-quadruplex adducts. In this study, deoxyguanosine/guanosine post-column addition experiments have been performed during HPLC-MS analysis of Achyrocline satureioides extract. The stabilities of the deoxyguanosine/guanosine adducts with 3-O-methylquercetin-7-O-glucoside, 4′-hydroxydehydrokawain-4′-O-glucoside, and 3,5-di-O-caffeoylquinic acid—compounds identified in the Achyrocline satureioides extract—have been tested by using collision-induced dissociation ‘in-source’. The obtained results show that the identified compounds form more stable adducts with deoxyguanosine and guanosine than the standards used for comparison, namely isoquercitrin and rutin. The performed molecular docking provided some insight into the structure of the adducts and revealed that multiple interactions are of key importance for their stabilities.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Biochemii i Analizy Żywności
dc.contributor.authorStężycka, Olga
dc.contributor.authorFrańska, Magdalena
dc.contributor.authorNowak, Damian
dc.contributor.authorHoffmann, Marcin
dc.contributor.authorKasperkowiak, Małgorzata
dc.contributor.authorBeszterda-Buszczak, Monika
dc.date.access2025-10-09
dc.date.accessioned2025-10-09T09:34:53Z
dc.date.available2025-10-09T09:34:53Z
dc.date.copyright2025-05-01
dc.date.issued2025
dc.description.abstract<jats:p>Polyphenols make a numerous and diverse group of plant secondary metabolites exhibiting remarkable anticancer activities, often attributed to their G-quadruplex binding properties. Therefore, there is a need to develop a high–throughput screening assay which would permit the evaluation of polyphenols’ binding properties toward G-quadruplex. As deoxyguanosine and guanosine are essential and key building blocks of G-quadruplexes, the stabilities of their adducts with polyphenols may reflect the stabilities of polyphenols–G-quadruplex adducts. In this study, deoxyguanosine/guanosine post-column addition experiments have been performed during HPLC-MS analysis of Achyrocline satureioides extract. The stabilities of the deoxyguanosine/guanosine adducts with 3-O-methylquercetin-7-O-glucoside, 4′-hydroxydehydrokawain-4′-O-glucoside, and 3,5-di-O-caffeoylquinic acid—compounds identified in the Achyrocline satureioides extract—have been tested by using collision-induced dissociation ‘in-source’. The obtained results show that the identified compounds form more stable adducts with deoxyguanosine and guanosine than the standards used for comparison, namely isoquercitrin and rutin. The performed molecular docking provided some insight into the structure of the adducts and revealed that multiple interactions are of key importance for their stabilities.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,9
dc.description.number9
dc.description.points140
dc.description.versionfinal_published
dc.description.volume26
dc.identifier.doi10.3390/ijms26094312
dc.identifier.issn1422-0067
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5332
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/26/9/4312
dc.languageen
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pagesart. 4312
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enguanosine adducts
dc.subject.enphenolics
dc.subject.enelectrospray ionization
dc.subject.enmass spectrometry
dc.subject.enpost-column addition
dc.subject.enliquid chromatography
dc.titlePost-Column Guanosine Addition as a Screening Tool in the Search for Effective G-Quadruplex Binders - A Case Study of Achyrocline satureioides Phenolic Compounds
dc.title.volumeSpecial Issue Recent Advancements in Natural and Synthetic DNA/RNA G-Quadruplex Binders
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue9
oaire.citation.volume26