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Excess nitrogen responsive HvMADS27 transcription factor controls barley root architecture by regulating abscisic acid level

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dc.abstract.enNitrogen (N) is an important element for plant growth and development. Although several studies have examined plants’ response to N deficiency, studies on plants’ response to excess N, which is common in fertilizer-based agrosystems, are limited. Therefore, the aim of this study was to examine the response of barley to excess N conditions, specifically the root response. Additionally, genomic mechanism of excess N response in barley was elucidated using transcriptomic technologies. The results of the study showed that barley MADS27 transcription factor was mainly expressed in the roots and its gene contained N-responsive cis-regulatory elements in the promoter region. Additionally, there was a significant decrease in HvMADS27 expression under excess N condition; however, its expression was not significantly affected under low N condition. Phenotypic analysis of the root system of HvMADS27 knockdown and overexpressing barley plants revealed that HvMADS27 regulates barley root architecture under excess N stress. Further analysis of wild-type (WT) and transgenic barley plants (hvmads27 kd and hvmads27 c-Myc OE) revealed that HvMADS27 regulates the expression of HvBG1 β-glucosidase, which in turn regulates abscisic acid (ABA) level in roots. Overall, the findings of this study showed that HvMADS27 expression is downregulated in barley roots under excess N stress, which induces HvBG1 expression, leading to the release of ABA from ABA-glucose conjugate, and consequent shortening of the roots.
dc.affiliationWydział Leśny i Technologii Drewna
dc.affiliation.instituteKatedra Chemii
dc.contributor.authorSmoczynska, Aleksandra
dc.contributor.authorPacak, Andrzej
dc.contributor.authorGrabowska, Aleksandra
dc.contributor.authorBielewicz, Dawid
dc.contributor.authorZadworny, Marcin [PAN - Kórnik]
dc.contributor.authorSingh, Kashmir
dc.contributor.authorDolata, Jakub
dc.contributor.authorBajczyk, Mateusz
dc.contributor.authorNuc, Przemyslaw
dc.contributor.authorKesy, Jacek
dc.contributor.authorWoźniak, Magdalena
dc.contributor.authorRatajczak, Izabela
dc.contributor.authorHarwood, Wendy
dc.contributor.authorKarlowski, Wojciech M.
dc.contributor.authorJarmolowski, Artur
dc.contributor.authorSzweykowska-Kulinska, Zofia
dc.date.access2026-02-18
dc.date.accessioned2026-02-18T12:01:58Z
dc.date.available2026-02-18T12:01:58Z
dc.date.copyright2022-09-12
dc.date.issued2022
dc.description.abstract<jats:p>Nitrogen (N) is an important element for plant growth and development. Although several studies have examined plants’ response to N deficiency, studies on plants’ response to excess N, which is common in fertilizer-based agrosystems, are limited. Therefore, the aim of this study was to examine the response of barley to excess N conditions, specifically the root response. Additionally, genomic mechanism of excess N response in barley was elucidated using transcriptomic technologies. The results of the study showed that barley MADS27 transcription factor was mainly expressed in the roots and its gene contained N-responsive <jats:italic>cis</jats:italic>-regulatory elements in the promoter region. Additionally, there was a significant decrease in <jats:italic>HvMADS27</jats:italic> expression under excess N condition; however, its expression was not significantly affected under low N condition. Phenotypic analysis of the root system of <jats:italic>HvMADS27</jats:italic> knockdown and overexpressing barley plants revealed that HvMADS27 regulates barley root architecture under excess N stress. Further analysis of wild-type (WT) and transgenic barley plants (<jats:italic>hvmads27 kd</jats:italic> and <jats:italic>hvmads27 c-Myc OE</jats:italic>) revealed that HvMADS27 regulates the expression of HvBG1 β-glucosidase, which in turn regulates abscisic acid (ABA) level in roots. Overall, the findings of this study showed that <jats:italic>HvMADS27</jats:italic> expression is downregulated in barley roots under excess N stress, which induces <jats:italic>HvBG1</jats:italic> expression, leading to the release of ABA from ABA-glucose conjugate, and consequent shortening of the roots.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if5,6
dc.description.points100
dc.description.versionfinal_published
dc.description.volume13
dc.identifier.doi10.3389/fpls.2022.950796
dc.identifier.issn1664-462X
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/7379
dc.identifier.weblinkhttps://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.950796/full
dc.languageen
dc.relation.ispartofFrontiers in Plant Science
dc.relation.pagesart. 950796
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enmiRNA
dc.subject.ennitrogen
dc.subject.enbarley
dc.subject.enroot architecture
dc.subject.enabscisic acid
dc.subject.enHvMADS27
dc.titleExcess nitrogen responsive HvMADS27 transcription factor controls barley root architecture by regulating abscisic acid level
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume13