Simple item page
 

Hyperosmolarity adversely impacts recombinant protein synthesis by Yarrowia lipolytica—molecular background revealed by quantitative proteomics

cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0001-8372-8459
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid6f5a4155-2edb-48cf-b362-f3180e151169
dc.abstract.enIn this research, we were interested in answering a question whether subjecting a Yarrowia lipolytica strain overproducing a recombinant secretory protein (rs-Prot) to pre-optimized stress factors may enhance synthesis of the rs-Prot. Increased osmolarity (3 Osm kg−1) was the primary stress factor implemented alone or in combination with decreased temperature (20 °C), known to promote synthesis of rs-Prots. The treatments were executed in batch bioreactor cultures, and the cellular response was studied in terms of culture progression, gene expression and global proteomics, to get insight into molecular bases underlying an awaken reaction. Primarily, we observed that hyperosmolarity executed by high sorbitol concentration does not enhance synthesis of the rs-Prot but increases its transcription. Expectedly, hyperosmolarity induced synthesis of polyols at the expense of citric acid synthesis and growth, which was severely limited. A number of stress-related proteins were upregulated, including heat-shock proteins (HSPs) and aldo–keto reductases, as observed at transcriptomics and proteomics levels. Concerted downregulation of central carbon metabolism, including glycolysis, tricarboxylic acid cycle and fatty acid synthesis, highlighted redirection of carbon fluxes. Elevated abundance of HSPs and osmolytes did not outbalance the severe limitation of protein synthesis, marked by orchestrated downregulation of translation (elongation factors, several aa-tRNA synthetases), amino acid biosynthesis and ribosome biogenesis in response to the hyperosmolarity. Altogether we settled that increased osmolarity is not beneficial for rs-Prots synthesis in Y. lipolytica, even though some elements of the response could assist this process. Insight into global changes in the yeast proteome under the treatments is provided.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.contributor.authorKubiak-Szymendera, Monika
dc.contributor.authorSkupien-Rabian, Bozena
dc.contributor.authorJankowska, Urszula
dc.contributor.authorCelińska, Ewelina
dc.date.access2026-01-26
dc.date.accessioned2026-01-26T07:37:16Z
dc.date.available2026-01-26T07:37:16Z
dc.date.copyright2021-12-16
dc.date.issued2022
dc.description.abstract<jats:sec> <jats:title>Abstract</jats:title> <jats:p>In this research, we were interested in answering a question whether subjecting a <jats:italic>Yarrowia lipolytica</jats:italic> strain overproducing a recombinant secretory protein (rs-Prot) to pre-optimized stress factors may enhance synthesis of the rs-Prot. Increased osmolarity (3 Osm kg<jats:sup>−1</jats:sup>) was the primary stress factor implemented alone or in combination with decreased temperature (20 °C), known to promote synthesis of rs-Prots. The treatments were executed in batch bioreactor cultures, and the cellular response was studied in terms of culture progression, gene expression and global proteomics, to get insight into molecular bases underlying an awaken reaction. Primarily, we observed that hyperosmolarity executed by high sorbitol concentration does not enhance synthesis of the rs-Prot but increases its transcription. Expectedly, hyperosmolarity induced synthesis of polyols at the expense of citric acid synthesis and growth, which was severely limited. A number of stress-related proteins were upregulated, including heat-shock proteins (HSPs) and aldo–keto reductases, as observed at transcriptomics and proteomics levels. Concerted downregulation of central carbon metabolism, including glycolysis, tricarboxylic acid cycle and fatty acid synthesis, highlighted redirection of carbon fluxes. Elevated abundance of HSPs and osmolytes did not outbalance the severe limitation of protein synthesis, marked by orchestrated downregulation of translation (elongation factors, several aa-tRNA synthetases), amino acid biosynthesis and ribosome biogenesis in response to the hyperosmolarity. Altogether we settled that increased osmolarity is not beneficial for rs-Prots synthesis in <jats:italic>Y. lipolytica</jats:italic>, even though some elements of the response could assist this process. Insight into global changes in the yeast proteome under the treatments is provided.</jats:p> </jats:sec><jats:sec> <jats:title>Key points</jats:title> <jats:p>• Temp enhances, but Osm decreases rs-Prots synthesis by Y. lipolytica.</jats:p> <jats:p>• Enhanced abundance of HSPs and osmolytes is overweighted by limited translation.</jats:p> <jats:p>• Global proteome under Osm, Temp and Osm Temp treatments was studied.</jats:p> </jats:sec>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if5,0
dc.description.number1
dc.description.points100
dc.description.versionfinal_published
dc.description.volume106
dc.identifier.doi10.1007/s00253-021-11731-y
dc.identifier.eissn1432-0614
dc.identifier.issn0175-7598
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/7113
dc.identifier.weblinkhttps://link.springer.com/article/10.1007/s00253-021-11731-y
dc.languageen
dc.relation.ispartofApplied Microbiology and Biotechnology
dc.relation.pages349–367
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.enYarrowia lipolytica
dc.subject.enheterologous protein
dc.subject.enproteomics of stress response
dc.titleHyperosmolarity adversely impacts recombinant protein synthesis by Yarrowia lipolytica—molecular background revealed by quantitative proteomics
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume106