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Antimicrobial and Antibiofilm Activity of Origanum vulgare Extracts Obtained by Supercritical Fluid Extraction Under Various Extraction Conditions

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dc.abstract.enSustainable management of agri-food product safety presents a major challenge requiring extensive action to ensure food safety and consumer health. The pursuit of environmentally friendly solutions that will constitute an alternative to the chemical compounds commonly used in agriculture and the food industries is one of the most important problems. One solution is plant extracts containing various biologically active compounds and exhibiting antimicrobial activity. This study aims to determine the biological activity of extracts obtained from Origanum vulgare L. (leaves) by supercritical CO2 (SC-CO2) extraction using different reaction conditions and compositions. In vitro studies revealed antimicrobial activity against selected bacteria (including Salmonella Enteritidis, Listeria monocytogenes, and Staphylococcus aureus) and fungi (Fusarium spp.), depending mainly on the microorganism species; however, extraction conditions also influenced these properties. The microscopic observations established by optical and fluorescence microscopy showed the changes in the fungal cell’s viability and morphology. There was no observed significant release of intracellular material as stated based on ICP-MS analysis of sodium and potassium concentration. Antibiofilm properties of extract obtained by extraction at 40 °C were also demonstrated against S. aureus, P. aeruginosa, and L. monocytogenes, with stronger properties observed against Gram-positive bacteria. Phytochemical characterization of the extracts was determined using a liquid chromatography system with an orbitrap mass spectrometer (LC/MS), identifying, i.e., phenolic acids: protocatechuic, hydroxybenzoic, caffeic, and rosmarinic; flavonoids: luteolin, naringenin, and kaempferol; and terpenoids: oleanolic and ursolic acids.
dc.affiliationWydział Leśny i Technologii Drewna
dc.affiliation.instituteKatedra Chemii
dc.contributor.authorGwiazdowska, Daniela
dc.contributor.authorWaśkiewicz, Agnieszka
dc.contributor.authorJuś, Krzysztof
dc.contributor.authorMarchwińska, Katarzyna
dc.contributor.authorFrąk, Szymon
dc.contributor.authorPopowski, Dominik
dc.contributor.authorPawlak-Lemańska, Katarzyna
dc.contributor.authorUwineza, Pascaline Aimee
dc.contributor.authorGwiazdowski, Romuald
dc.contributor.authorPadewska, Daria
dc.contributor.authorRoszko, Marek
dc.contributor.authorBryła, Marcin
dc.date.access2025-04-23
dc.date.accessioned2025-07-17T12:27:11Z
dc.date.available2025-07-17T12:27:11Z
dc.date.copyright2024-12-10
dc.date.issued2024
dc.description.abstract<jats:p>Sustainable management of agri-food product safety presents a major challenge requiring extensive action to ensure food safety and consumer health. The pursuit of environmentally friendly solutions that will constitute an alternative to the chemical compounds commonly used in agriculture and the food industries is one of the most important problems. One solution is plant extracts containing various biologically active compounds and exhibiting antimicrobial activity. This study aims to determine the biological activity of extracts obtained from Origanum vulgare L. (leaves) by supercritical CO2 (SC-CO2) extraction using different reaction conditions and compositions. In vitro studies revealed antimicrobial activity against selected bacteria (including Salmonella Enteritidis, Listeria monocytogenes, and Staphylococcus aureus) and fungi (Fusarium spp.), depending mainly on the microorganism species; however, extraction conditions also influenced these properties. The microscopic observations established by optical and fluorescence microscopy showed the changes in the fungal cell’s viability and morphology. There was no observed significant release of intracellular material as stated based on ICP-MS analysis of sodium and potassium concentration. Antibiofilm properties of extract obtained by extraction at 40 °C were also demonstrated against S. aureus, P. aeruginosa, and L. monocytogenes, with stronger properties observed against Gram-positive bacteria. Phytochemical characterization of the extracts was determined using a liquid chromatography system with an orbitrap mass spectrometer (LC/MS), identifying, i.e., phenolic acids: protocatechuic, hydroxybenzoic, caffeic, and rosmarinic; flavonoids: luteolin, naringenin, and kaempferol; and terpenoids: oleanolic and ursolic acids.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,6
dc.description.number24
dc.description.points140
dc.description.versionfinal_published
dc.description.volume29
dc.identifier.doi10.3390/molecules29245823
dc.identifier.issn1420-3049
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/3894
dc.identifier.weblinkhttps://www.mdpi.com/1420-3049/29/24/5823
dc.languageen
dc.relation.ispartofMolecules
dc.relation.pagesart. 5823
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.enphytochemical characterization
dc.subject.ensupercritical fluid extraction
dc.subject.enbiological activity
dc.subject.enCO2 extracts
dc.subject.enoregano
dc.subject.enantimicrobial and antibiofilm activity
dc.titleAntimicrobial and Antibiofilm Activity of Origanum vulgare Extracts Obtained by Supercritical Fluid Extraction Under Various Extraction Conditions
dc.title.volumeSpecial Issue Advances in Natural Products and Their Biological Activities
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue24
oaire.citation.volume29