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Identification and Analysis of Candidate Genes Associated with Yield Structure Traits and Maize Yield Using Next-Generation Sequencing Technology

cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0001-9516-8911
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cris.virtual.author-orcid0000-0002-0102-0084
cris.virtual.author-orcid0000-0002-0153-4624
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cris.virtual.author-orcid0000-0003-4445-5624
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cris.virtualsource.author-orcid2faa4bbb-a129-4bec-8137-bd8eeedf40e0
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cris.virtualsource.author-orcid51a5a68b-106b-4e9d-bd9b-79d15d3ec0c1
cris.virtualsource.author-orcid4ddc81ce-066b-4d2e-a9f3-015a6c34a525
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cris.virtualsource.author-orcid36fc7bfc-89c8-4648-af52-05dd31161d2f
dc.abstract.enThe main challenge of agriculture in the 21st century is the continuous increase in food production. In addition to ensuring food security, the goal of modern agriculture is the continued development and production of plant-derived biomaterials. Conventional plant breeding methods do not allow breeders to achieve satisfactory results in obtaining new varieties in a short time. Currently, advanced molecular biology tools play a significant role worldwide, markedly contributing to biological progress. The aim of this study was to identify new markers linked to candidate genes determining grain yield. Next-generation sequencing, gene association, and physical mapping were used to identify markers. An additional goal was to also optimize diagnostic procedures to identify molecular markers on reference materials. As a result of the conducted research, 19 SNP markers significantly associated with yield structure traits in maize were identified. Five of these markers (28629, 28625, 28640, 28649, and 29294) are located within genes that can be considered candidate genes associated with yield traits. For two markers (28639 and 29294), different amplification products were obtained on the electrophorograms. For marker 28629, a specific product of 189 bp was observed for genotypes 1, 4, and 10. For marker 29294, a specific product of 189 bp was observed for genotypes 1 and 10. Both markers can be used for the preliminary selection of well-yielding genotypes.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Genetyki i Hodowli Roślin
dc.affiliation.instituteKatedra Metod Matematycznych i Statystycznych
dc.affiliation.instituteKatedra Technologii Żywności Pochodzenia Roślinnego
dc.contributor.authorNowak, Bartosz
dc.contributor.authorTomkowiak, Agnieszka
dc.contributor.authorSobiech, Aleksandra
dc.contributor.authorBocianowski, Jan
dc.contributor.authorKowalczewski, Przemysław Łukasz
dc.contributor.authorSpychała, Julia
dc.contributor.authorJamruszka, Tomasz
dc.date.access2025-05-05
dc.date.accessioned2025-07-25T10:49:20Z
dc.date.available2025-07-25T10:49:20Z
dc.date.copyright2023-12-29
dc.date.issued2024
dc.description.abstract<jats:p>The main challenge of agriculture in the 21st century is the continuous increase in food production. In addition to ensuring food security, the goal of modern agriculture is the continued development and production of plant-derived biomaterials. Conventional plant breeding methods do not allow breeders to achieve satisfactory results in obtaining new varieties in a short time. Currently, advanced molecular biology tools play a significant role worldwide, markedly contributing to biological progress. The aim of this study was to identify new markers linked to candidate genes determining grain yield. Next-generation sequencing, gene association, and physical mapping were used to identify markers. An additional goal was to also optimize diagnostic procedures to identify molecular markers on reference materials. As a result of the conducted research, 19 SNP markers significantly associated with yield structure traits in maize were identified. Five of these markers (28629, 28625, 28640, 28649, and 29294) are located within genes that can be considered candidate genes associated with yield traits. For two markers (28639 and 29294), different amplification products were obtained on the electrophorograms. For marker 28629, a specific product of 189 bp was observed for genotypes 1, 4, and 10. For marker 29294, a specific product of 189 bp was observed for genotypes 1 and 10. Both markers can be used for the preliminary selection of well-yielding genotypes.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,8
dc.description.number1
dc.description.points100
dc.description.versionfinal_published
dc.description.volume15
dc.identifier.doi10.3390/genes15010056
dc.identifier.issn2073-4425
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/3977
dc.identifier.weblinkhttps://www.mdpi.com/2073-4425/15/1/56
dc.languageen
dc.relation.ispartofGenes
dc.relation.pagesart. 56
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enmaize
dc.subject.ennext-generation sequencing (NGS)
dc.subject.enassociation mapping
dc.subject.enyield structure traits
dc.subject.enyield
dc.subject.enSNP markers
dc.subject.enSilicoDArT markers
dc.titleIdentification and Analysis of Candidate Genes Associated with Yield Structure Traits and Maize Yield Using Next-Generation Sequencing Technology
dc.title.volumeSpecial Issue Advances in Genetics and Genomics of Plants
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume15