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Multidimensional discriminant analysis of species, strains and culture age of closely related entomopathogenic fungi using reflectance spectroscopy

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cris.virtual.author-orcid0000-0002-3512-1350
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cris.virtualsource.author-orcid884ee7cf-babf-4820-934b-f49db8b5e1b5
dc.abstract.enThe diversity of fungal strains is influenced by genetic and environmental factors, growth conditions and mycelium age, and the spectral features of fungal mycelia are associated with their biochemical, physiological, and structural traits. This study investigates whether intraspecific differences can be detected in two closely related entomopathogenic species, namely Cordyceps farinosa and Cordyceps fumosorosea, using ultraviolet A to shortwave infrared (UVA-SWIR) reflectance spectra. Phylogenetic analysis of all strains revealed a high degree of uniformity among the populations of both species. The characteristics resulting from variation in the species, as well as those resulting from the age of the cultures were determined. We cultured fungi on PDA medium and measured the reflectance of mycelia in the 350–2500 nm range after 10 and 17 days. We subjected the measurements to quadratic discriminant analysis (QDA) to identify the minimum number of bands containing meaningful information. We found that when the age of the fungal culture was known, species represented by a group of different strains could be distinguished with no more than 3–4 wavelengths, compared to 7–8 wavelengths when the age of the culture was unknown. At least 6–8 bands were required to distinguish cultures of a known species among different age groups. Distinguishing all strains within a species was more demanding: at least 10 bands were required for C. fumosorosea and 21 bands for C. farinosa. In conclusion, fungal differentiation using point reflectance spectroscopy gives reliable results when intraspecific and age variations are taken into account.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Entomologii i Ochrony Środowiska
dc.contributor.authorJasiewicz, Jarosław
dc.contributor.authorPiekarczyk, Jan
dc.contributor.authorStępień, Łukasz
dc.contributor.authorTkaczuk, Cezary
dc.contributor.authorSosnowska, Danuta
dc.contributor.authorUrbaniak, Monika
dc.contributor.authorRatajkiewicz, Henryk
dc.date.accessioned2025-01-22T12:12:59Z
dc.date.available2025-01-22T12:12:59Z
dc.date.issued2024
dc.description.bibliographybibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,3
dc.description.number15 May 2024
dc.description.points140
dc.description.volume313
dc.identifier.doi10.1016/j.saa.2024.124135
dc.identifier.eissn1873-3557
dc.identifier.issn1386-1425
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2398
dc.languageen
dc.relation.ispartofSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
dc.relation.pagesart. 124135
dc.rightsClosedAccess
dc.sciencecloudsend
dc.subject.enhyperspectral data
dc.subject.enfungal mycel
dc.subject.enpigmens
dc.subject.enchemometrics
dc.subject.engenetic variation
dc.subject.enoptical classification
dc.titleMultidimensional discriminant analysis of species, strains and culture age of closely related entomopathogenic fungi using reflectance spectroscopy
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume313