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Multi-faceted analysis of bacterial transformation of nitrofurantoin

cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-9477-8563
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cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid73a2e591-ad92-47ae-b1e4-3eb06ca9d58f
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enExcessive presence of antibiotics and their residues can be dangerous to the natural environment. To reduce this negative effect, efficient strategies to remove them from the ecosystem are required. This study aimed to explore the potential of bacterial strains to degrade nitrofurantoin (NFT). Single strains isolated from contaminated areas, namely Stenotrophomonas acidaminiphila N0B, Pseudomonas indoloxydans WB, and Serratia marcescens ODW152 were employed in this study. Degradation efficiency and dynamic changes within the cells during NFT biodegradation were investigated. For this purpose, atomic force microscopy, flow cytometry, zeta potential, and particle size distribution measurements were applied. Serratia marcescens ODW152 showed the highest performance in removal of NFT (96 % in 28 days). The AFM images revealed modifications of cell shape and surface structure induced by NFT. Zeta potential showed significant variations during biodegradation. Cultures exposed to NFT had a broader size distribution than the control cultures due to increased cells agglomeration or aggregation. 1-Aminohydantoin and semicarbazide were detected as nitrofurantoin biotransformation products. They showed increased cytotoxicity toward bacteria as determined by spectroscopy and flow cytometry. Results of this study suggest that nitrofurantoin biodegradation leads to formation of stable transformation products that significantly affect the physiology and structure of bacterial cells.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.contributor.authorPacholak, Amanda
dc.contributor.authorJuzwa, Wojciech
dc.contributor.authorZgoła-Grześkowiak, Agnieszka
dc.contributor.authorKaczorek, Ewa
dc.date.accessioned2025-10-31T12:33:36Z
dc.date.available2025-10-31T12:33:36Z
dc.date.issued2023
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if8,2
dc.description.number20 May 2023
dc.description.points200
dc.description.volume874
dc.identifier.doi10.1016/j.scitotenv.2023.162422
dc.identifier.eissn1879-1026
dc.identifier.issn0048-9697
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5676
dc.languageen
dc.relation.ispartofScience of the Total Environment
dc.relation.pagesart. 162422
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enatomic force microscopy
dc.subject.enbiotransformation
dc.subject.enmetabolites
dc.subject.ensingle cell microbiology
dc.subject.entoxicity
dc.titleMulti-faceted analysis of bacterial transformation of nitrofurantoin
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume874